5-氮胞苷联合丹酚酸B促进大鼠骨髓间充质干细胞向心肌样细胞分化

5-Azacytidine combined with salvianolic acid B can promote differentiation from bone marrow Mesenchymal stem cells derived from rats to cardiomyocytes

关键词:  5-氮胞苷  丹酚酸B  骨髓间充质干细胞  分化

Key words:  5-Azacytidine  Salvianolic acid B  MSCs  differentiation

摘要:

[目的] 探讨5-氮胞苷及丹酚酸B的联合作用下骨髓间充质干细胞(MSCs)分化为心肌细胞的能力。[方法] 体外分离、培养、鉴定的骨髓MSCs分为4组:正常对照组,5-氮胞苷组,丹酚酸B组,5-氮胞苷+丹酚酸B组。诱导2周后,利用实时荧光定量PCR(QPCR)和蛋白免疫印迹技术检测心肌早期转录因子(GATA-4)和心肌特异性肌凝蛋白重链(α-MHC)的表达含量。[结果] 各诱导组GATA-4及α-MHC RNA和蛋白的表达量均高于正常对照组,而5-氮胞苷+丹酚酸B组高于5-氮胞苷组和丹酚酸B组,且差异有统计学意义。[结论] 5-氮胞苷联合丹酚酸B可提高MSCs向心肌样细胞的分化率。

Abstract:

[Objective] To discuss the role of 5-Azacytidine and salvianolic acid B in inducing differentiation of Mesenchymal stem cells (MSC) derived from rats into cardiomyocytes. [Methods] A method was established by culturing MSCs from the bone marrow of rat. The well-conditioned MSCs were divided into four groups: the control group, the 5-Azacytidine (5-aza) group, the salvianolic acid B (salB) group, the 5-Azacytidine combined with salvianolic acid B (5-aza+salB) group. Two weeks after inducing the mRNA and protein expression of GATA-4 and α-MHC were detected by QPCR and Western Blot in each group. [Results] The mRNA and protein expression of GATA-4 and α-MHC in each induced group were higher than control group, and the 5-aza+salB group was significantly higher than 5-aza and salB group. [Conclusion] 5? -Azacytidine combined with Salvianolic acid B can promote differentiation rate of cardiomyocytes from Mesenchymal stem cells of bone marrow derived from rats.