| 摘要: |
| [目的] 建立芒果叶中芒果苷含量的测定方法;测定不同时期芒果叶中芒果苷的含量。[方法] 采用高效液相色谱法(HPLC),Cosmosil ODS柱分离,流动相:乙腈-1%冰醋酸溶液(15:85)为流动相,流速1.0 mL/min,柱温25 ℃,检测波长254 nm.从海南采集不同时期的芒果叶,测定其中芒果苷含量。[结果] 芒果苷在0.069 5～2.224 0 μg范围内进样量与峰面积呈良好的线性关系,平均回收率96.77 %,RSD=1.19 %.[结论] 所建立的芒果苷含量测定方法灵敏度高,准确度高,重现性好。 |
| 关键词: 芒果叶 芒果苷 高效液相色谱法 含量测定 |
| DOI：10.11656/j.issn.1673-9043.2013.01.12 |
| 分类号: |
| 基金项目:国家自然科学基金项目(81173524);国家科技重大专项项目-重大新药创新(2012X09103201031);天津市科技创新体系及条件平台建设计划项目(10SYSYJC289000);教育部新世纪优秀人才计划项目(NCET-10-0958) |
|
| Determination of Mangiferin content in mango tree leaves |
|
GUO Ling-ling, ZHANG Yi, LIU Er-wei, HAN Li-feng, WANG Tao
|
|
Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin, Tianjin 300193, China
|
| Abstract: |
| [Objective] To establish the determination method of Mangiferin content in mango tree leaves for analyzing samples collected in different season. [Methods] HPLC method was performed on Cosmosil ODS column. Mobile phase: CH3CN-1% HAc (15:85), flow rate: 1 mL/min, column temperature: 25 ℃, detection wavelength: 254 nm. The samples of mango tree leaves were collected in different seasonin from Hainan province for determination of mangiferin. [Results] The method showed a linear relationship between peak area and injection amount at the range of 0.069 5～2.224 0 μg for mangiferin. The average recovery rate was 96.77%, RSD=1.19%. [Conclusion] This HPLC method for the content determination of mangiferin shows good sensitivity, accuracy and repeatability. |
| Key words: mango tree leave Mangiferin HPLC determination |
