| 摘要: |
| [目的] 研究心脑舒通及其单体成分对小胶质细胞（BV-2）的影响及其作用机制. [方法] 实验分为对照组、脂多糖刺激组、加药组，检测心脑舒通（不同稀释倍数的心脑舒通及其单体成分）对小胶质细胞活力及脂多糖（LPS）诱导小胶质细胞一氧化氮（NO）产生的影响. [结果] 心脑舒通对LPS诱导的小胶质细胞NO的产生无明显抑制作用，在心脑舒通13种单体成分中，伪原薯蓣皂苷、支脱皂苷元、薯蓣皂苷元可以在不影响细胞活力的情况下抑制NO产生. [结论] 心脑舒通部分单体成分抑制激活小胶质细胞NO的产生可能是心脑舒通发挥神经保护作用机制之一. |
| 关键词: 心脑舒通 一氧化氮 炎性介质 小胶质细胞 |
| DOI：10.11656/j.issn.1673-9043.2014.01.07 |
| 分类号: |
| 基金项目:重大新药创制（2011ZX09201-201）. |
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| Effect of Xinnao Shutong and its monomer on Lipopolysaccharides activated microglia cells |
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LU Guo-yan, YANG Hong-yun, WANG Shao-xia, HU Li-min
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Key Laboratory of Tianjin University of TCM, State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Chinese medicine Pharmacology, Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin 300193, China
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| Abstract: |
| [Objective] To study the effects of Xinnao Shutong and its monomer composition on microglia (BV-2) and its mechanism. [Methods] The experiment was divided into control group, lipopolysaccharide(LPS) group, dosing group. The effect of Xinnao Shutong (different dilution multiples of Xinnao Shutong and its monomer composition) on cellular activity and the production of nitric oxide (NO) in microglia cell induced by lipopolysaccharide (LPS). [Results] Xinnao Shutong could not significantly inhibit the microglia in releasing NO induced by LPS. Among the 13 kinds of monomer composition of Xinnao Shutong, Pseudoprotodioscin, Gitogenin, Diosgenin could inhibit NO secretion without affecting the cell vitality. [Conclusion] Some monomer composition of Xinnao Shutong can inhibit the releasing of NO in activated microglia. It may be one of the mechanisms of neural protection. |
| Key words: Xinnao Shutong NO inflammatory mediators microglial cell |
