高浓度丹参注射液对高糖所致内皮细胞损伤的保护作用

Protective effect of Danshen injection in high concentration on endothelial cells injured by high glucose

关键词:  丹参注射液  内皮细胞  保护

Key words:  Danshen injection  endothelial cell  protection

摘要:

[目的] 探讨丹参注射液对高糖所致血管内皮细胞损伤的保护作用。[方法] 采用0.1%Ⅰ型胶原酶消化灌注脐静脉获得脐静脉内皮细胞，将第2代细胞悬液放入96孔培养板进行培养，待细胞近融合时分别换成不同的培养液，即：正常对照组、高糖损伤组和不同浓度丹参注射液保护组，作用48 h后四甲基偶氮唑蓝（MTT）比色法测细胞存活数量。[结果] 10-1、10-2、10-3 g/L 3种浓度的丹参保护液均能改善高糖对内皮细胞损伤的影响（P<0.05）,10-4 g/L浓度的丹参保护液不能改善高糖对内皮细胞损伤的影响无统计学意义（P>0.05）。[结论] 高浓度的丹参注射液对高糖损伤的血管内皮细胞有保护作用。

Abstract:

[Objective] To explore the effect of Danshen injection on human vascular endothelial cells injured by high glucose. [Methods] The endothelial cells were obtained by perfusion digestion of blood from umbilical vein using type Ⅰ collagenase (0.1%), and the second passage of endothelial cells was incubated in 96 plate. The formed subconfluent, endothelial cells were incubated with different medium respectively: normal control, group inured with high glucose and protected group with different concentration of Danshen injection. 48 hours later, the number of survived cells were determined with MTT. [Results] At the concentration of 10^-1, 10^-2, 10^-3 mg/ml, Danshen injection could improve the protective effect against the injury of endothelial cells harmed by high glucose(P<0.05), at the concentration of 10^-4 mg/ml could not improve the protective effect (P>0.05). [Conclusion] Danshen injection of high concentration can protect endothelial cells harmed by high glucose.