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| 两种黄芩苷眼用制剂防治硒性白内障作用研究 |
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韩真真1,2, 李楠1,2, 郭丽丽1,2, 佟玲1,2, 陈静1,2
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1.天津市现代中药重点实验室, 天津中医药大学, 天津300193;2.天津中医药大学, 现代中药发现与制剂技术教育部工程研究中心, 天津300193
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| 摘要: |
| [目的] 考察黄芩苷眼用固体脂质纳米粒（BA-SLNs）、黄芩苷眼用固体脂质纳米粒凝胶（BA-SLNsG）抗硒性白内障作用。[方法] 采用12 日龄Wistar乳鼠制造硒性白内障模型。给药组眼内分别滴入20 μL治疗药物，空白组和模型组分别给予同体积生理盐水，阳性组给予同体积白内停，每天给药4 次，持续8 d，观察每日大鼠晶状体变化。测定超氧化物歧化酶活性（SOD）、谷胱甘肽（郧杂匀）含量并采用蛋白免疫印迹方法初步考察两种黄芩苷眼用制剂对α A-晶状体蛋白（α A-Crystallin）表达的影响。[结果] 两给药组大鼠晶状体浑浊程度明显轻于模型组。空白组和给药组SOD 活性明显高于模型组（P<0.01）；各组GSH含量也高于模型组（P<0.05）。蛋白免疫印迹结果表明模型组晶状体中α A-Crystallin 表达量均高于其余各组。[结论] BA-SLNs、BA-SLNsG均能有效提高晶状体抗氧化能力，下调α A-Crystallin 表达，延缓白内障发病，可进一步开发成临床药物。 |
| 关键词: 硒性白内障 黄芩苷 超氧化物歧化酶 谷胱甘肽 蛋白免疫印迹 |
| DOI：10.11656/j.issn.1673-9043.2014.03.10 |
| 分类号: |
| 基金项目:教育部新世纪优秀人才支持计划（NCET-12-1068）；天津市应用基础及前沿技术研究计划（12JCYBJC18700）。 |
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| Preventive effect of two ophthalmic preparations of baicalin against seleniumcataract |
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HAN Zhen-zhen1,2, LI Nan1,2, GUO Li-li1,2, TONG Ling1,2, CHEN Jing1,2
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1.Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To study the anti-selenite cataract effect of two ophthalmic preparations of Baicalin, Baicalin-loaded solid lipid nanoparticles (BA-SLNs) and Baicalin-loaded solid lipid nanoparticles in gel (BA-SLNsG). [Methods] The selenite cataract model was reproduced on Wistar rats of 12-days old. 20 μL of two ophthalmic solutions/eye drops were given to the treatment groups (TP), meanwhile same volume of saline was given to blank group (BP) and model group (MP), same volume of pirenoxine sodiumwas given to control group (CP). Drugs were delivered 4 times a day for 8 days. The change of lens was observed daily. The expression of α A-Crystallin was tested by Western blotting. The antioxidant activity was determined by superoxide disproportionation enzymes and glutathione. [Results] The degree of lens opacity of a TP was significantly lighter than that of MP. SOD vitality of in BP and TP were significantly higher than MP (P<0.01);GSH content of BP and control group were significantly higher than MP (P<0.01). BA-SLNs group and BA-SLNsG group were higher than model group(P<0.05). The expression of α A-Crystallin in model group was higher than others. [Conclusion] Both BA-SLNs and BA-SLNsG can improve the antioxidant capacity of crystalline lens by regulating down α A-Crystallin expression. It shows that the possibility of the application of BA-SLNs and BA-SLNsG into clinical. |
| Key words: seleniumcataract baicalin SOD GSH Western blotting |
