| 摘要: |
| [目的]对国槐（Sophora japonica L.）米70%乙醇提取物的化学成分继续研究，为开发利用该植物资源提供依据。[方法]采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及高效液相色谱法等方法进行分离纯化，并通过理化性质及光谱分析鉴定化合物的结构。[结果]从国槐米体积分数为70%的乙醇提取物中分离鉴定了6个单体化合物，分别为icariside F2（1）、gallic acid 3-O-β-D-（6’-O-galloyl）-glucopyranoside（2）、ethyl-α-L-rhamnopyranosyl（1→6）-β-D-glucoside（3）、Et-（6-O-p-hydroxybenzoyl）-β-D-glucopyranoside（4）、胸腺嘧啶脱氧核苷（5）和左旋色氨酸（6）.[结论]化合物 1～5为首次从槐属植物中分离得到的化合物。 |
| 关键词: 槐米 化学成分 结构鉴定 |
| DOI：10.11656/j.issn.1673-9043.2014.04.11 |
| 分类号: |
| 基金项目:国家自然基金项目资助（81202995）；国家科技重大专项项目-重大新药创制（2011ZX09307-002-01）；新世纪优秀人才支持计划资助（NCET-12-1069）. |
|
| Isolation and identification of chemical constituents from Flos Sophorae I |
|
LIU Li-li, LI Xiao-xia, CHEN Yue, SHI Ping-ping, QU Lu, ZHANG Yi
|
|
Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin, Tianjin 300193, China
|
| Abstract: |
| [Objective] To study the chemical constituents from Flos Sophorae.[Methods] The chemical components were obtained by using chromatographies such as silica gel, ODS, Sephadex LH-20 and HPLC, and identified by the chemical and physical methods, especially spectral analysis.[Results] Six compounds were obtained and identified as icariside F2 (1), gallic acid 3-O-β-D-(6'-O-galloyl)glucopyranoside (2), ethyl-α-L-rhamnopyranosyl(1→6)-β-D-glucoside (3), Et (6-O-p-hydroxybenzoyl)-β-D-glucopyranoside (4), L-tryptophan (5) and 2'-deoxythymidine (6), respectively.[Conclusion] Compounds 1～5 were obtained from Sophora genus firstly. |
| Key words: Flos Sophorae chemical constituents structural identification |
