丹红注射液减轻DPPH所致离体心脏功能损伤的作用研究

Protective effects of Danhong injection on DPPH-induced injuries in the isolated hearts of rats

关键词:  丹红注射液  二苯基三硝基苯肼  离体心脏  心功能损伤

Key words:  Danhong injection  DPPH  isolated hearts  Langendorff

摘要:

[目的] 在大鼠离体心脏灌流系统上观察丹红注射液对二苯基三硝基苯肼（DPPH）自由基所致心功能损伤的保护作用。[方法] 大鼠离体心脏采用Langendorff 灌流，基础平衡30 min后进行各组预给药10 min后，用含0.25 μmol/L DPPH的K-H液灌流心脏10 min，造成心脏功能损伤，最后用K-H液灌流10 min进行恢复。记录灌注压（PP）、左心室舒张压（LVEDP）、左心室收缩压（LVSP）、心率（HR）、左心室内压最大上升速率（+dp/dt_max）、左心室内压最大下降速率（-dp/dt_max），并计算心率压力乘积（RPP）.[结果] 丹红注射液灌注10 min可以显着降低灌注压，扩张冠状动脉。DPPH自由基损伤后RPP、+dp/dt_max显着降低，-dp/dt_max显着升高。用含丹红注射液的K-H液预处理10 min，丹红2.5 μL/mL组、丹红5 μL/mL组可以减轻DPPH自由基导致的心脏功能损伤。[结论] 在离体心脏模型中丹红注射液能扩张冠状动脉，减轻外源性自由基对心脏功能的损伤。

Abstract:

[Objective] To examine the protective effects of Danhong injection on DPPH-induced myocardial injuries in the isolated hearts of rats in Langendorff model. [Methods] The isolated hearts of rats were perfused in a Langendorff model for 30 min and pre-treatment with Danhong injection for 10 min. The hearts were subjected to Krebs-Henseleit(K-H)solution with DPPH 0.25 μmol/L for 10 min to cause myocardial dysfunction, and followed by 10 min recovery with K-H solution. The perfusion pressure(PP), left ventricular systolic pressure(LVSP), left ventricular end diastolic pressure (LVEDP), heart rate (HR), maximal and minimum rate of pressure development(±dp/dt_max)were recorded. And the heart rate pressure product (RPP)was calculated. [Results] The perfusion pressure significantly decreased after 10 min perfusion with Danhong injection. Perfusion with DPPH causes a remarkable decrease in RPP, +dp/dt_max and an increase in -dp/dt_max. Pretreatment with Danhong injection of 2.5 μL/mL and 5 μL/mL for 10 min significantly reduced the DPPH-induced myocardial injuries. [Conclusion] Danhong injection can dilate coronary artery and protect against the myocardial injuries induced by DPPH in isolated rat hearts.