| 摘要: |
| [目的] 研究荷叶中的化学成分, 为进一步开发利用荷叶资源提供依据。[方法] 采用正相硅胶、反相ODS、SephadexLH-20等柱色谱及制备型高效液相色谱法(HPLC)等方法进行分离纯化, 并通过理化性质、质谱、核磁以及文献比对等方法, 对化合物的结构进行鉴定。[结果] 从荷叶体积分数为70%的乙醇提取物中分离鉴定了6个化合物, 分别为:sarmentol F (1);(3S, 5R, 6S, 7E, 9R)-7-megastigmene-3, 6, 9-triol (2);(3S, 5R, 6R, 7E)-3, 5, 6-trihydroxy-7-megastigmen-9-one (3);3S, 5R-dihydroxy-6R, 7-mestigmadien-9-one (4);3S, 5R-dihydroxy-6S, 7-mestigmadien-9-one (5);R-(-)-去氢催吐萝芙叶醇(6).[结论] 化合物1～6 均为首次从莲属植物中分离得到。 |
| 关键词: 荷叶 化学成分 结构鉴定 |
| DOI：10.11656/j.issn.1673-9043.2015.02.08 |
| 分类号: |
| 基金项目:天津市高等学校科技发展基金一般资助项目(20120203);高等学校博士学科点专项科研基金(20121210110007). |
|
| Isolation and identification of chemical constituents from Nelumbinis Folium(Ⅲ) |
|
XIA Ming-hui1, PENG Shuang2, ZHAO Jing1, HAN Li-feng1, ZHANG Yi1, WANG Tao1
|
|
1.Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin, Tianjin 300193, China;2.Qinghai Lake Pharmaceutical Company Limited, Xining 810000, China
|
| Abstract: |
| [Objective] To investigate the chemical constituents of the lotus leaf for futher research and development. [Methods] The chemical components were obtained by using several chromatographies, such as silica gel, ODS, Sephadex LH-20 and PHPLC, and their structures were identified by the physico-chemical methods, MS, NMR spectra and comparing with literatures. [Results] Six compounds were isolated from 70 % ethanol extract of lotus leaves and identified as sarmentol F (1); (3S, 5R, 6S, 7E, 9R)-7-megastigmene-3,6,9-triol (2); (3S, 5R, 6R, 7E)-3, 5, 6-trihydroxy-7-megastigmen-9-one (3); 3S, 5R-dihydroxy-6R, 7-mestigmadien-9-one (4); 3S, 5R-dihydroxy-6S, 7-mestigmadien-9-one(5), and R-(-)-dehydrovomifoliol (6). [Conclusion] Compounds 1～6 are obtained from Nelumbo Adans. for the first time. |
| Key words: Nelumbinis Folium chemical constituents structure identification |
