| 摘要: |
| [目的] 正交实验法优选柴胡桂枝颗粒处方药材的提取工艺。[方法] 以柴胡皂苷a、芍药苷、黄芩苷及出膏率为检测指标, 采用正交实验法优选柴胡等药材的乙醇回流提取工艺。[结果] 柴胡桂枝颗粒乙醇回流提取的最佳提取工艺为10倍量80%乙醇提取3次, 每次提取2 h.[结论] 优选的柴胡桂枝颗粒提取工艺稳定可行, 适合于工业化大生产。 |
| 关键词: 柴胡桂枝颗粒 正交实验 提取工艺 柴胡皂苷a 黄芩苷 芍药苷 |
| DOI：10.11656/j.issn.1673-9043.2015.02.11 |
| 分类号: |
| 基金项目:国家“十二五”重大新药创制专项课题(2014ZX09 101 021). |
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| Optimization of extraction technology for Chaihu Guizhi granule by multi-index comprehensive evaluation method |
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HAN Zhong-yao1, ZHOU Fu-jun2, LIU Fang3, HOU Wen-Bin2
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1.Qiannan Medical College for Nationalities, Duyun 558000, China;2.Tianjin Institute of Pharmaceutical Research, Tianjin 3001933, China;3.First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To optimize extraction process of prescription drugs from Chaihu Guizhi Granule by orthogonal test.[Methods] Ethanol extraction technology of Radix bupleurl and other herbs was optimized by orthogonal test with Saikosaponin A, baicalin, paeoniflorin and extract rate as detection index.[Results] Optimum extraction process conditions were determined as follows: Radix bupleurl and other herbs were extracted 3 times with 2 h by 10 times the amount of 80% ethanol.[Conclusion] This optimized extraction technology of Chaihu Guizhi Granule was stable and feasible, it could be extended to industrial production. |
| Key words: Chaihu Guizhi granule orthogonal test extraction technology Saikosaponin A baicalin paeoniflorin |
