温阳通窍中药复方对大鼠脑胶质瘤作用机制的体内实验研究

In vivo study of traditional Chinese medicine Wenyang Tongqiao effect on rat Glioma mechanism

关键词:  C6脑胶质瘤  温阳通窍中药复方  SDF-1  CTGF  bFGF

Key words:  C6 glioma  Wenyang Tongqiao  SDF-1  CTGF  bFGF

摘要:

[目的]研究温阳通窍中药复方对大鼠C6脑胶质瘤的抑制作用机制。[方法] C6胶质瘤细胞大鼠颅内接种, 建立脑胶质瘤模型。磁共振成像(MRI)条件下将模型动物随机分为对照组、温阳通窍复方低剂量组和高剂量组。除对照组外, 其余各组给予对应剂量的药物, 3周后取脑胶质瘤组织, 进行病理组织学检测并进行相关分析, 检测基质细胞衍生因子-1(SDF-1)、结缔组织生长因子(CTGF)、碱性成纤维细胞生长因子(bFGF)指标。[结果] MRI检测表明体内实验成功构建大鼠脑胶质瘤模型。组织病理学观察结果显示, 与对照组动物相比, 给药组动物肿瘤微血管减少, 可见变性坏死瘤细胞, 瘤组织坏死较对照组明显, 高剂量治疗组略强。温阳通窍给药组SDF-1、CTGF、bFGF较对照组呈降低趋势。[结论] 温阳通窍中药复方对C6脑胶质瘤细胞的增殖确有抑制作用, 该作用或许与肿瘤组织中SDF-1、CTGF、bFGF的表达相关。

Abstract:

[Objective] To study Wenyang Tongqiao traditional Chinese herbal compound inhibition on rat C6 glioma mechanism. [Methods] Put C6 glioma cells in rats with intracranial inoculation, and establish glioma model. Under the MRI (magnetic resonance imaging) conditions the animals were randomly divided into a control group model, Wenyang Tongqiao compound low dose and high dose group. In addition to the control group, the rest of the group was given the corresponding doses of the drug. After three weeks, extract glioma tissue, and use histopathological detection and correlation analysis to detect SDF-1 (stromal cell-derived factor 1), CTGF (connective tissue growth factor), bFGF (basic fibroblast growth factor) indicators. [Results] MRI detected that successfully rat glioma model in vivo tests is constructed. Histopathological observation showed that, compared with control animals, tumor microvessels of animals administered reduce. Visible necrosis of tumor cells, tumor necrosis are more obvious compared with the control group, and the high dose group was slightly stronger. Wenyang Tongqiao administration group SDF-1, CTGF, bFGF compared with the control group showed a decreasing trend.[Conclusion] Wenyang Tongqiao Chinese herbal compound on C6 glioma cell proliferation inhibition indeed, perhaps related to the role of the SDF-1, CTGF, bFGF expression in tumor tissue.