| 摘要: |
| [目的] 研究血栓通及其单体成分对小胶质细胞(BV-2)的影响及其作用机制。[方法] 实验分为对照组、脂多糖刺激组、加药组和阳性药米诺环素组,CCK-8检测血栓通(不同稀释倍数的血栓通及其单体成分)对小胶质细胞活力的影响;Greiss法检测脂多糖(LPS)诱导小胶质细胞一氧化氮(NO)产生的影响,实时定量聚合酶链反应(PCR)检测炎性基因的表达。[结果] 血栓通在0.5~50 mg/L范围内对LPS诱导的小胶质细胞NO的产生没有抑制作用;在血栓通5种单体成分中,人参皂苷Rg1、Rb1、Re、三七皂苷对小胶质细胞NO的产生没有抑制作用;人参皂苷Rd在不影响细胞活力的情况下能明显抑制NO产生,并且能抑制炎症基因诱导型一氧化氮合酶(iNOS)及白介素-1β(IL-1β)、白介素-6(IL-6) mRNA 的表达。[结论] 血栓通单体成分人参皂苷Rd抑制激活的小胶质细胞产生NO和iNOS 、IL-1β、IL-6 mRNA的表达可能是血栓通发挥神经保护作用的物质基础。 |
| 关键词: 血栓通 一氧化氮 炎性介质 小胶质细胞 |
| DOI：10.11656/j.issn.1673-9043.2015.06.07 |
| 分类号: |
| 基金项目:国家重大新药创制项目资助(2012ZX09101201-004). |
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| Effect of Xueshuantong and Ginsenoside Rd on lipopolysaccharides actived microglia cells |
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YANG Hong-yun, LIU Xiao-lei, CHAI Li-juan, WANG Shao-xia, HU Li-min
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Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To study the effects of Xueshuantong and its monomer on actived microglia (BV-2) and its mechanism. [Methods] The experiment was divided into control group, lipopolysaccharide(LPS) group, dosing group and positive control group (mino), detecting of Xueshuantong (different dilution multiples of Xueshuantong and its monomer composition) to the vitality of microglia and nitric oxide(NO) induced by LPS using the Greiss method. [Results] Between 0.5~50 μg/mL, Xueshuantong could not inhibit the microglia release NO induced by LPS, among the 5 kinds of monomer composition of Xueshuantong, ginsenoside Rg1, Rb1, Re and Notoginsenoside could not inhibit the microglia release NO induced by LPS, however, ginsenoside Rd could inhibit NO secretion without affecting the cell vitality, and inhibit the mRNA expression of iNOS, IL-1β, IL-6. [Conclusion] The monomer composition of ginsenoside Rd in Xueshuangtong can inhibit activated microglia release NO and the mRNA of iNOS,IL-1β,IL-6 significantly. It may be one of neural protection mechanism. |
| Key words: Xueshuantong nitric oxide inflammatory mediator microglia |
