延胡索药材及饮片的UPLC质量控制方法

The UPLC quality control method of Corydalis Rhizamo herbs and herbal pieces

关键词:  延胡索  UPLC  延胡索乙素  去氢紫堇碱

Key words:  Corydalis Rhazamo  UPLC  tetrahydropalmatine  dehydrocorydaline

摘要:

[目的] 检测延胡索药材及饮片中有效成分的含量,建立延胡索药材和饮片的超高液相色谱法(UPLC)质量控制方法。[方法] 采用UPLC检测方法,ACQUITY UPLC BEH C₁₈色谱柱(100 mm×2.1 mm,1.7 μm),乙腈(B)-0.1%甲酸水溶液,调pH值至5.0(A),流速:0.3 mL/min,柱温:45 ℃,进样量:5 μL;梯度洗脱。以延胡索乙素和去氢紫堇碱的含量为指标对延胡索的水提液进行检测。[结果] 样品中延胡索乙素的含量在0.229 1～0.775 5 mg/g之间,去氢紫堇碱的含量在0.781 0～2.931 0 mg/g之间,不同样品延胡索乙素和去氢紫堇碱的含量差异很大。[结论] UPLC法的灵敏度更高、检测速度更快,适用于组分多、成分复杂的中药材的分析及质量的评价。

Abstract:

[Objectiye] To develop a UPLC quality control method of Corydalis Rhizamo herbs and herbal pieces by determining the contents of active components.[Methods] The separation was performed on an ACQUITY UPLC BEH C₁₈(100 mm×2.1 mm, 1.7 μm) column in the UPLC method. The mobile phases consisted of acetonitrile (B) and 0.1% formic acid aqueous solution(A, pH=5.0) with gradient elution. The flow rate and column temperature were set at 0.3 mL/min and 45 ℃, respectively. The injection volume was 5 μL. The contents of tetrahydropalmatine and dehydrocorydaline were taken as indexes for the water extract of corydalis rhizoma.[Results] The content of tetrahydropalmatine was in the range of 0.229 1~0.775 5 mg/g and that of dehydrocorydaline was 0.781 0~2.931 0 mg/g. The contents of tetrahydropalmatine and dehydrocorydaline varied greatly in different samples of Corydalis Rhizamo. [Conclusion] Tetrahydropalmatine and dehydrocorydaline were stable in the water extract, which could be used as the index components for the quality control of Corydalis Rhizamo. The UPLC method was fast, efficient and of higher sensitivity. It was suitable for the rapid detection of active components and the quality evaluation of traditional Chinese medicines.