| 摘要: |
| [目的] 研究小花鬼针草对金黄色葡萄球菌、大肠杆菌、白色念珠菌、铜绿假单胞菌的体外抗菌活性。[方法] 采用50%乙醇-石油醚-乙酸乙酯提取，并以大孔树脂AB-8柱层析富集小花鬼针草中总黄酮；以K-b药敏实验考察总黄酮的抗菌效果，倍比稀释法测定其最低抑菌浓度（MIC）。[结果] 小花鬼针草总黄酮对大肠杆菌敏感性较差，对白色念珠菌、铜绿假单胞菌、金黄色葡萄球菌有一定抑制作用。[结论] 小花鬼针草总黄酮部位对多种菌表现出抑制作用，提示可能有较广的抗菌谱。 |
| 关键词: 小花鬼针草 黄酮 最低抑菌浓度 金黄色葡萄球菌 铜绿假单胞菌 |
| DOI：10.11656/j.issn.1673-9043.2018.03.16 |
| 分类号:R285.6 |
| 基金项目: |
|
| The antibacterial activity of total Flavonoids in Bidensbipinnata |
|
CAO Mingchen1, FANG Mengxiang1, HUANG Xin2
|
|
1.Shandong University of Traditional Chinese Medicine, Jinan 250355, China;2.Shandong Qianfoshan Hospital, Jinan 250014, China
|
| Abstract: |
| [Objective] To study the in vitro antibacterial activity of Bidensbipinnata to Staphylococcus aureus, Escherichia coli, Candida albicans and Pseudomonas aeruginosa.[Methods] The total flavonoids were extracted by 50% ethanol-petroleum ether-ethyl acetate and purified by macroporous resin AB-8 column chromatography. The drug susceptibility of bacteria of total flavonoids was measured by disk diffusion method, the minimum inhibitory concentration (MIC) was determined by afar dilution method.[Results] The total flavonoids of Bidensbipinnata were less sensitive to Escherichia coli and had a certain inhibitory effect on Candida albicans, Pseudomonas aeruginosa and Staphylococcus aureus.[Conclusions] The total flavonoids of Bidensbipinnata showed inhibitory effects on a variety of bacteria, suggesting that there may be a broad antibacterial spectrum. |
| Key words: Bidensbipinnata flavonoids minimum inhibitory concentration Staphylococcus aureus Pseudomonas aeruginosa |
