小茴香挥发油对小鼠胃排空和肠推进的影响

Effects of essential oil of fennel on gastric emptying and small bowel peristalsis in mice

关键词:  小茴香挥发油  胃排空  肠推进  甲硫酸新斯的明

Key words:  fennel  gastri emptying  small bowel peristalsis  neostigmine

摘要:

[目的]研究小茴香挥发油对在体小鼠胃肠运动及对家兔大鼠离体肠管收缩活动的影响。[方法]1）小鼠在体实验：腹腔注射硫酸阿托品建立小鼠胃肠运动功能障碍模型，甲硫酸新斯的明腹腔注射建立小鼠胃排空和肠推进亢进模型，营养性半固体糊灌胃法观察小茴香挥发油对正常小鼠及上述两种模型小鼠小肠推进率和胃排空率的变化。2）离体肠道平滑肌实验：使用PowerLab15T生物信号采集系统观察小茴香挥发油对大鼠和家兔离体肠管运动的影响。[结果]小茴香挥发油不同浓度可以抑制正常小鼠的肠推进及甲硫酸新斯的明所致的肠推进亢进（P<0.05）；但对甲硫酸阿托品所致的小鼠胃肠运动抑制模型作用不明显，小茴香挥发油对大鼠和家兔离体小肠运动的频率和张力有极显著的抑制作用（P<0.01）。[结论]小茴香挥发油具有抑制小鼠胃肠动力的作用，其作用机制可能是通过拮抗乙酰胆碱的作用，从而缓解胃肠道痉挛，为今后小茴香的制剂开发及临床用药的提供理论依据。

Abstract:

[Objective] To study the effects of essential oil of fennel on gastric emptying and small bowel peristalsis in mice in vivo and contraction activity of the smooth muscle of rabbits and rats in vitro.[Methods] 1)Mice in vivo experiment:establishment of gastrointestinal motility disorder model by intraperitoneal injection of atropine in mice and establishment of gastric emptying and intestinal propulsion model by intraperitoneal injection of neostigmine in mice. Observation on the changes of intestinal propelling rate and gastric emptying rate of fennel essential oil on the above two model mice by feeding with nutritional semisolid paste. 2)Isolated intestinal smooth muscle experiment:the effects of essential oil of fennel on the movement of isolated intestine in rats and rabbits and its mechanism were studied by PowerLab15T biological signal acquisition system.[Results] Different concentrations of essential oil of fennel could inhibit intestinal propulsive and neostigmine induced hyperactivity in normal mice (P<0.05). And it has a significant inhibitory effect on the movement of rabbit small intestine in vitro as well (P<0.01).[Conclusion] Fennel essential oil has inhibitory effect on gastrointestinal motility in mice by enhancing cholinesterase activity. This study can provide theoretical basis for the preparation development and clinical use of fennel in the future.