| 摘要: |
| [目的]建立淫羊藿药材超高效液相色谱（UPLC）指纹图谱分析方法。[方法]采用Agilent 1290超高效液相色谱仪，以CORTECS® C18（2.1 mm×100 mm，1.6 μm）色谱柱，乙腈-0.1%甲酸水进行梯度洗脱，流速为0.3 mL/min，柱温为40℃，进样量为1 μL，检测波长为270 nm。[结果]12个不同批次淫羊藿药材有21个共有峰，其中有6个已知峰，分别为木兰花碱、朝藿定A、朝藿定B、朝藿定C、淫羊藿苷和宝藿苷I，不同批次的样品与标准指纹图谱相似度均在0.911以上。[结论]本实验建立的UPLC指纹图谱分析方法稳定、精密度高，可应用于淫羊藿药材的质量控制和评价。 |
| 关键词: 淫羊藿 超高效液相色谱 指纹图谱 |
| DOI：10.11656/j.issn.1673-9043.2019.05.17 |
| 分类号:R917 |
| 基金项目:国家自然科学基金项目（81673824）；重大新药创制项目（2015ZX09501004-003-004）。 |
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| UPLC fingerprint of Epimedium herbs |
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YIN Yanwei1, JIA Qi1, MA Wenjuan1, OUYANG Huizi2, CHANG Yanxu1, HE Jun1
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1.Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] Establishing UPLC fingerprint analysis method for Epimedium medicinal materials.[Methods] Gradient elution with a CORTECS® C18 column (2.1 mm×100 mm,1.6 μm) in acetonitrile-0.1% formic acid water using an Agilent 1 290 series ultra performance liquid chromatography with a flow rate of 0.3 mL/min and a column temperature of 40℃,the injection volume is 1 μL,and the detection wavelength is 270 nm.[Results] There are 21 common peaks in 12 batches of different kinds of epimedium medicinal herbs,including 6 known peaks,namely magnoflorine,epimedin A,epimedin B,epimedin C,icariin and baohuoside I,the similarity between different batch samples and standard fingerprints is above 0.911.[Conclusion] The UPLC fingerprint analysis method established in this experiment is stable and has high precision,and can be applied to the quality control and evaluation of epimedium. |
| Key words: epimedium UPLC fingerprint |
