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| 叶酸修饰载丹参酮ⅡA纳米结构脂质载体的制备及其理化表征 |
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刘静静1,2, 李新悦1,2, 吴玉梅1,2, 马丽1,2, 张兵1,2, 刘志东1,2, 李佳玮1,2
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1.天津中医药大学, 现代中药发现与制剂技术教育部工程中心, 天津 301617;2.天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 301617
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| 摘要: |
| [目的] 制备载丹参酮ⅡA纳米结构脂质载体，将含有叶酸的PEG链配体修饰于制剂表面获得叶酸修饰载丹参酮ⅡA纳米结构脂质载体（FA-TanⅡA-NLC），使其具有肿瘤靶向作用，并对制剂进行理化表征。[方法] 采用乳化蒸发-低温固化法制备制剂，以粒径、电位和包封率等为指标对制剂进行初步评价，采用透射电子显微镜（TEM）、差示扫描量热法（DSC）和X射线衍射法（XRD）对制剂进行进一步的表征。[结果] 制备的叶酸修饰载丹参酮ⅡA纳米结构脂质载体（FA-TanⅡA-NLC）粒径为（19.14±0.43）nm，PDI为0.424±0.01，电位为（-11.67±0.25）mV，DSC以及XRD结果均表明丹参酮ⅡA以无定型形式被包载于纳米结构脂质载体中。[结论] 本研究制得的FA-TanⅡA-NLC粒径分布均一，体系稳定，包封率高。 |
| 关键词: 丹参酮ⅡA 纳米结构脂质载体 叶酸 |
| DOI：10.11656/j.issn.1673-9043.2020.01.21 |
| 分类号:R283 |
| 基金项目: |
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| Preparation and physicochemical characterization of folic acid modified nanostructured lipid carrier loaded with tanshinone ⅡA |
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LIU Jingjing1,2, LI Xinyue1,2, WU Yumei1,2, MA Li1,2, ZHANG Bing1,2, LIU Zhidong1,2, LI Jiawei1,2
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1.Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-Established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
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| Abstract: |
| [Objective] The nanostructured lipid carrier (NLC) loaded with tanshinone ⅡA was prepared and modified by inserting the DSPE-PEG-FA on its surface,and the physicochemical characterizations of the NLC were investigated.[Methods] The FA-TanⅡA-NLC was prepared by emulsification-evaporation technique. The particle size,zeta potential and encapsulation efficiency were used to preliminarily evaluate the NLC,which was further characterized by TEM,DSC and XRD.[Results] The particle diameter of FA-Tan ⅡA-NLC was of (19.14±0.43) nm, PDI was 0.424±0.01,and the zeta potential was of (-11.67±0.25) mV. The encapsulation efficiency was 99%. The results of DSC and XRD indicated that tanshinone ⅡA was encapsulated in the nanostructured lipid carrier in an amorphous form.[Conclusion] The FA-TanIIA-NLC prepared in this experiment had uniform particle size distribution,stable potential and high encapsulation efficiency. |
| Key words: tanshinone ⅡA nanostructure lipid carrier folic acid |
