| 摘要: |
| [目的] 观察祛湿通络方含药血清对肺炎支原体（MP）感染的RAW264.7细胞肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）表达的影响。[方法] 1）祛湿通络方含药血清的制备：SD大鼠随机分为空白对照组、中药低、中、高剂量组、阿奇霉素组，各组均连续给药7 d。2）MP感染的RAW264.7细胞炎症模型建立：模型组和各给药组分别加入MP菌液100 μL，空白对照组加入100 μL DMEM完全培养液，置于培养箱中培养2 h。3）酶联免疫吸附（ELISA）法检测祛湿通络方对MP感染的RAW264.7细胞TNF-α、IL-6表达的影响。[结果] 与空白对照组比较，MP感染的RAW264.7细胞TNF-α、IL-6的分泌均显著上升（P<0.05）；与模型组比较，各给药组对TNF-α、IL-6均有不同程度的下调作用（P<0.05），其中阿奇霉素组对TNF-α、IL-6的调节作用最优，中药低、中、高剂量组呈现剂量依赖性。[结论] 祛湿通络方对MP感染的RAW264.7细胞TNF-α、IL-6的高表达具有下调作用，且呈剂量依赖性，这可能是祛湿通络方减轻炎症反应的机制之一。 |
| 关键词: 肺炎支原体 祛湿通络方 RAW264.7细胞 TNF-α IL-6 |
| DOI：10.11656/j.issn.1673-9043.2020.05.19 |
| 分类号:R285.5 |
| 基金项目:山东省重点研发计划（2016GSF202043）。 |
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| Effect of Qushi Tongluo Formulation on TNF-α and IL-6 expression in RAW264.7 infected with mycoplasma pneumoniae |
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LI Na1, YU Guangzhi1, ZHANG Baoqing2, WANG Xiao2
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1.Department of Pediatrics, Affiliated Hospital of Jining Medical College, Jining 272029, China;2.Department of Pediatrics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, China
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| Abstract: |
| [Objective] To observe the effect of Qushi Tongluo Formulation on TNF-α and IL-6 expression in RAW264.7 cells infected with mycoplasma pneumoniae (MP).[Methods] 1)Preparation of drug serum containing Qushi Tongluo Formulation:SD rats were randomly divided into blank control group,low,medium and high dose group of traditional Chinese medicine,and azithromycin group. Each group received continuous administration for 7days. 2) Establishment of MP-infected RAW264.7 cell inflammation model:model group and each treatment group were added with 100 μL of MP bacterial fluid,and blank control group was added with 100 μL of DMEM complete culture fluid,and cultured in incubator for 2 h. 3) The effect of Qushi Tongluo Formulation on the expression of TNF-aand IL-6 in MP infected RAW264.7 cells was detected by ELISA.[Results] Compared with the blank control group,the secretion of TNF-α and IL-6 in MP-infected RAW264.7 cells increased significantly (P<0.05). Compared with the model group,TNF-α and IL-6 were down-regulated in all groups (P<0.05).[Conclusion] Qushi Tongluo Formulation can downregulate the high expression of TNF-α and IL-6 in MP infected RAW264.7 cells in a dose-dependent manner,which may be one of the mechanisms by which Qushi Tongluo Formulation can reduce inflammation. |
| Key words: mycoplasma pneumoniae Qushi Tongluo Formulation RAW264.7 cell TNF-α IL-6 |
