基于UPLC-Q-TOF-MS/MS分析藜芦中化学成分

Chemical component analysis of Veratrum by UPLC-Q-TOF-MS/MS

关键词:  藜芦  超高效液相色谱-飞行时间质谱联用技术  化学成分

Key words:  Veratrum  UPLC-Q-TOF-MS/MS  chemical constituent

摘要:

[目的] 采用超高效液相色谱-飞行时间质谱联用技术（UPLC-Q-TOF-MS/MS）对藜芦中的化学成分进行研究。[方法] 采用CORTECS C₁₈色谱柱（2.1 mm×100 mm，1.6 μm），以0.1%甲酸水溶液（A）-乙腈（B）为流动相进行梯度洗脱，柱温40℃，流速为0.3 mL/min。质谱采用电喷雾（ESI）离子源，正、负离子监测模式。利用Agilent Masshunter Qualitative Analysis分析软件对所得信息进行处理，结合Metlin数据库、对照品图谱及相关文献，对各化学成分进行辨识。[结果] 共鉴定推测出藜芦中包括生物碱类、黄酮类、有机酸类等47个化学成分。[结论] 通过UPLC-Q-TOF-MS/MS建立了一种能准确、简便、快速分析藜芦中化学成分的方法，为深入研究藜芦的药效物质基础和优选藜芦质量控制指标提供了理论依据。

Abstract:

[Objective] To analyze the main chemical constituents of traditional Chinese medicine compound Veratrum by using UPLC-Q-TOF-MS/MS technology.[Methods] Using CORTECS C₁₈ chromatographic column (2.1 mm×100 mm,1.6 μm) with 0.1% formic acid aqueous solution (A) and acetonitrile (B) as mobile phase for gradient elution. The flow rate was 0.3 mL/min,the column temperature was 40℃. The mass spectrometer uses an electrospray ion source (ESI) to detect in positive and negative ion modes respectively. The compounds were identified by the processed information of Agilent Masshunter Qualitative Analysis software,as well as Metlin database,the literature and reference substance.[Results] A total of forty-seven chemical components in Veratrum were identified and predicted,including alkaloids,flavonoids,organic acids,and other types.[Conclusion] A simple,rapid,and accurate method for analyzing the chemical components in Veratrum was established,which provides a theoretical basis for elucidating the medicinal material basis and optimizing the quality control indicators of Veratrum.