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| 甘草地上部分不同提取方法提取物成分HPLC-MS/MS分析 |
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张玥辉1, 薄颖异2, 王文全1,3,4, 常冠华1, 王文地1, 崔洁3, 侯俊玲1,4, 谭卫忠5
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1.北京中医药大学中药学院, 北京 102488;2.天津市宝坻区人民医院药剂科, 天津 301800;3.中国医学科学院北京协和医学院药用植物研究所, 北京 100193;4.中药材规范化生产教育部工程研究中心, 北京 102488;5.国药控股济南有限公司, 济南 250100
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| 摘要: |
| [目的] 对甘草地上部分的水提物、醇提物和CO2超临界萃取物中黄酮类成分的组成进行分析。[方法] 高效液相色谱法（HPLC）Agilent SB-C18柱，乙腈-0.5%甲酸水梯度洗脱，体积流量1.0 mL/min，柱温30℃，进样量10 μL，检测波280 nm。质谱使用电喷雾离子源，负离子模式。[结果] 在水提物中共定性出9个化合物，主要为黄酮（醇）苷类物质；在醇提物中共定性出14个化合物，主要为黄酮（醇）苷元类物质和异戊烯基黄酮类物质；在CO2超临界萃取物中共定性出10个化合物，主要为异戊烯基黄酮类物质。[结论] 鉴定出甘草地上部分不同提取方法提取物中有不同性质的黄酮类物质，可以为后续提取富集研究提供依据。 |
| 关键词: 甘草地上部分 HPLC-MS/MS分析 黄酮类物质 |
| DOI：10.11656/j.issn.1673-9043.2021.02.04 |
| 分类号:R284.2 |
| 基金项目:国家重点研发计划资助项目（2018YFC1706500）。 |
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| Analysis on chemical constituents from extracts of different extraction methods of aerial parts of Glycyrrhiza uralensis by HPLC-MS/MS |
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ZHANG Yuehui1, BO Yingyi2, WANG Wenquan1,3,4, CHANG Guanhua1, WANG Wendi1, CUI Jie3, HOU Junling1,4, TAN Weizhong5
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1.School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China;2.Pharmacy Department, Tianjin Baodi Hospital, Tianjin 301800, China;3.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China;4.Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs, Ministry of Education, Beijing 102488, China;5.Sinopharm Group Jinan Co., Ltd., Jinan 250100, China
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| Abstract: |
| [Objective] To analyze chemical constituents from water extract,ethanol extract and CO2 supercritical extract of aerial parts of Glycyrrhiza uralensis.[Methods] HPLC uses Agilent SB-C18 analytical column,acetonitrile-0.5% formic acid solution gradient elution,volume flow rate 1.0 mL/min,column temperature 30℃,injection volum 10 μL under 280 nm detection wavelength. Mass spectrometry uses electrospray ion source,negative ion mode.[Results] In water extract,9 compounds were identified mainly consists of flavonoids (alcohols) glycosides. A total of 14 compounds were identified in ethanol extract,mainly flavonoids (alcohols) aglycones and isopentenyl flavone. In CO2 supercritical extract,10 compounds were identified,mainly isopentenyl flavone.[Conclusion] Extracts of different extraction methods with different properties are acquired providing basis for further research. |
| Key words: aerial parts of Glycyrrhiza uralensis HPLC-MS/MS analysis flavonoids |
