金沸草HS-GC-MS指纹图谱研究

HS-GC-MS fingerprint of Inulae Herba

关键词:  金沸草  静态顶空  气质联用  指纹图谱

Key words:  Inulae Herba  head-space  gas chromatography-mass spectrometry  fingerprint

摘要:

[目的] 基于静态顶空-气相质谱联用（HS-GC-MS）技术分析金沸草挥发性化学成分，构建其特征性指纹图谱，为金沸草的质量评价及药效物质基础研究提供依据。[方法] 采用HS-GC-MS技术构建22批金沸草挥发性成分指纹图谱，确定共有峰，利用中药色谱指纹图谱相似度评价系统对不同批次样品质量差异进行评价。[结果] 建立了金沸草药材HS-GC-MS指纹图谱，定性出104个化学成分，主要为萜类、醛类等；22批药材所含挥发性成分既有整体相似性又有特征差异性，共获得并鉴定13个共有峰，主要为萜烯类、醛类及醇类等。[结论] 研究建立的HSGC-MS指纹图谱分析方法稳定，可为金沸草的质量评价及控制奠定基础。

Abstract:

[Objective] To establish head-space-gas chromatography(HS-GC-MS) fingerprint analysis method for quality control of Inulae Herba.[Methods] The volatile components and the common peaks are identified by head-space(HS) combined with gas chromatography-mass spectrometry(GC-MS) in 22 batches of Inulae Herba from different origins. The similarity evaluation system was used to evaluate the quality difference of Inulae Herba from different origins.[Results] Finally,the 104 chemical constituents are identified,mainly including terpenes,aldehydes and other types. There are similarities and differences in the components of 22 batches of samples. The 13 common peaks including terpenes,aldehydes,alcohols and other chemical components are identified by GC-MS in 22 batches of Chinese herbal medicine.[Conclusion] The established HS-GC-MS fingerprint analysis method is stable and can provide the basis for the quality control and evaluation of Inulae Herba.