| 摘要: |
| [目的] 联合网络药理学和代谢组学分析针灸治疗缺血性脑卒中的作用机制。[方法] 借助Swiss Target Prediction数据库、Gene Cards数据库查找作用靶点;Cytoscape 3.9.1软件构建药物-成分-靶点网络;STRING数据库获得PPI网络图;最后利用David平台进行KEGG信号通路富集分析。采用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF/MS)分析各组大鼠代谢轮廓变化,借助Metabo Analyst数据库查询相关代谢通路。[结果] 假手术组和模型组共筛选出49个基于血清的代谢生物标志物(P＜0.05),主要代谢途径为甘油磷脂代谢、亚油酸代谢、α-亚麻酸代谢、乙醚脂质代谢、赖氨酸降解、花生四烯酸代谢,其中包含的代谢产物主要是磷脂酰胆碱、溶血磷脂酰胆碱、脂肪酸及磷脂酰乙醇胺。[结论] 针灸治疗可能通过多靶点、多通路来改善脑血管微循环、促进血管生成、减少脂质沉积、减缓炎症反应等改善机体免疫。结合文献数据,认为针灸的治疗具有组分多、目标多、路径多的特点,对于缺血性脑卒中有一定疗效。 |
| 关键词: 缺血性脑卒中 针灸 代谢组学 网络药理学 分子对接 |
| DOI：10.11656/j.issn.1673-9043.2023.06.10 |
| 分类号:R743.32 |
| 基金项目:国家重点研发计划项目（2022YFB4703104）；国家自然科学基金资助项目（81903938，81904295）。 |
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| Exploration of the mechanism of acupuncture treatment for ischemic stroke based on network pharmacology and metabolomics |
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ZHANG Yintao1, LIU Xinyu2, WANG Yuyu2, GUO Tao3, ZHANG Fangfang2, QIU Jiwen3, WANG Yuming2
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1.Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;3.College of Acupuncture and Tuina, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
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| Abstract: |
| [Objective] Combined with network pharmacology and metabolomics,the mechanism of acupuncture and moxibustion in the treatment of ischemic stroke was analyzed.[Methods] We use the Swiss Target Prediction database and Gene Cards database to search for the target of action;Construct a drug component target network using Cytoscape 3.9.1 software;STRING database obtains PPI network;Finally,the David platform was used for KEGG signaling pathway enrichment analysis. Ultra high-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF/MS) was used to analyze the metabolic profile changes of rats in each group,and relevant metabolic pathways were queried using the Metabo Analyst database.[Results] A total of 49 serum based metabolic biomarkers were selected from the sham surgery group and model group (P＜0.05),with the main metabolic pathways being phospholipid metabolism,linoleic acid metabolism α- Linolenic acid metabolism,ether lipid metabolism,lysine degradation,arachidonic acid metabolism,which mainly contain metabolites such as phosphatidylcholine,Lys phosphatidylcholine,fatty acids,and phosphatidylethanolamine.[Conclusion] Acupuncture and moxibustion therapy may improve cerebral vascular microcirculation,promote angiogenesis,reduce lipid deposition,slow down inflammatory reaction,and improve body immunity through multiple targets and pathways. Combined with literature data,it is believed that acupuncture and moxibustion treatment has the characteristics of multiple components,multiple goals,and multiple paths,and has a certain effect on ischemic stroke. |
| Key words: ischemic stroke acupuncture metabolomics network pharmacology molecular docking |
