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| 基于UPLC-Q-Exactive Orbitrap-MS的经典名方温胆汤化学成分分析 |
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张奎斌1,2, 邢斌1,2, 宋纹3, 王仁兴1,2, 张青青1,2, 刘志东1,2, 皮佳鑫1,2
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1.天津中医药大学组分中药国家重点实验室, 天津 301617;2.天津中医药大学现代中药发现与制剂技术教育部工程中心, 天津 301617;3.天津宏仁堂药业有限公司, 天津 301617
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| 摘要: |
| [目的] 采用超高效液相色谱-四极杆静电场轨道阱高分辨质谱法（UPLC-Q-Exactive Orbitrap-MS）对温胆汤的化学成分进行快速识别和鉴定。[方法] 采用Waters Acquity BEH C18（2.1×100 mm，1.7 μm），以0.1%甲酸水溶液（A）-乙腈（B）作为流动相进行梯度洗脱，流速为0.3 mL/min、柱温30℃；采用加热电喷雾离子源（HESI），正、负离子模式同时检测，扫描模式为Full MS/dd-MS2，扫描范围m/z 100~1 500。[结果] 通过与对照品比对、精确相对分子质量、多级质谱碎片信息并结合文献报道、质谱数据库信息等对化合物进行鉴别。从温胆汤中共鉴别了121个成分，包括70个黄酮类成分，18个香豆素类成分，9个皂苷类成分，10个生物碱成分，3个苯丙素类成分及其他类化合物11个。其中15个成分通过与对照品比对后确认。[结论] 利用UPLC-Q-Exactive Orbitrap-MS技术较为系统地阐明了温胆汤的物质基础，可为该复方药效物质基础的深入研究和质量标准的合理制定奠定基础。 |
| 关键词: 温胆汤 UPLC-Q-Exactive Orbitrap-MS 成分分析 经典名方 |
| DOI：10.11656/j.issn.1673-9043.2024.02.05 |
| 分类号:R284.1 |
| 基金项目: |
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| Analysis of chemical constituents in Wendan Decoction based on UPLC-Q-Exactive Orbitrap-MS |
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ZHANG Kuibin1,2, XING Bin1,2, SONG Wen3, WANG Renxing1,2, ZHANG Qingqing1,2, LIU Zhidong1,2, PI Jiaxin1,2
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1.State Key Laboratory of Component Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;3.Tianjin Hongrentang Pharmaceutical Co., Ltd, Tianjin 300122, China
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| Abstract: |
| [Objective] To rapidly identify the chemical components of Wendan Decoction by UPLC-Q-Exactive Orbitrap-MS. [Method] Waters Acquity BEH C18(2.1×100 mm,1.7 m) was used for gradient elution with 0.1% formic acid aqueous solution(A)-Acetonitrile(B) as mobile phase in positive and negative ion modes. The flow rate was 0.3 mL/min,the column temperature was 30℃,and the injection volume was 2 L. Adopting heated electrospray ion source(HESI),scan mode is Full MS/dd-MS2 and scan range m/z 100~1500. [Results] The compound was identified by comparing with the accurate relative molecular mass,multi-stage mass spectrometry fragment information,combining with literature reports and mass database information. A total of 121 ingredients were identified from Wendan Decoction,including 70 flavonoids,18 coumarins,9 saponins,11 nitrogen-containing ingredients,3 phenylpropanoids and 10 other components. Among them,15 components were confirmed after comparing with the reference substance. [Conclusion] UPLC-Q-Exactive Orbitrap-MS technology was used to systematically clarify the basic ingredients of Wendan Decoction,which can lay a foundation for the better research of the pharmacodynamic material basis and the reasonable formulation of quality standards. |
| Key words: Wendan Decoction UPLC-Q-Exactive Orbitrap-MS component analysis ancient classic Chinese medicine formula |
