阳离子化白及多糖的党参抗皮肤衰老凝胶的制备与评价

Preparation and evaluation of anti-skin aging gel of codonopsis pilosula based on cationic bletilla polysaccharide

关键词:  党参水提物  激活蛋白-1转录活性  抗皮肤衰老  阳离子化白及多糖  卡波姆  水凝胶

Key words:  water extract of codonopsis pilosula  transcriptional activity of AP-1  anti-skin aging  ationic bletilla polysaccharide  kapom  hydrogel

摘要:

[目的] 构建一种基于阳离子化白及多糖（CBSP）的新型稳定的党参水提物凝胶用于抗皮肤衰老。[方法] 采用水提法制备党参提取物，通过研究党参水提物对激活蛋白-1（AP-1）转录活性的影响，探究其抗皮肤衰老作用。并制备一种用于局部递送党参水提物的凝胶，首先采用 Williamson 合成法制备CBSP，通过傅里叶红外光谱（FTIR），电位，氮取代度（DS）等检测对其进行表征，后将其与卡波姆（CP）通过静电作用制备复合凝胶基质（CBSP-CP），评价其稳态黏度、流变曲线和生物相容性等，分别采用CBSP-CP凝胶基质和3种CP市售凝胶基质与党参水提物结合制备党参水提物凝胶。[结果] 党参水提物可以显著抑制AP-1的转录活性，具有一定的抗皮肤衰老作用；FTIR，电位，DS结果显示成功制备了CBSP；CBSP-CP复合凝胶基质具有良好的流变性和生物相容性，CBSP-CP党参水提物凝胶与3种CP市售党参水提物凝胶比较在黏度方面具有更好的稳定性。[结论] 该研究为开发高稳定的中药党参提取物凝胶剂提供了实验室数据与参考，也为中药党参提取物应用于抗皮肤衰老类产品提供了途径。

Abstract:

[Objective] To construct a new stable aqueous extract gel of Radix Codonopsis based on cationic Bletilla striata polysaccharide（CBSP） for anti-skin aging. [Methods] The aqueous extract of Codonopsis pilosula was prepared by water extraction. The effect of the aqueous extract of Codonopsis pilosula on the transcription activity of activated protein-1（AP-1） was studied to explore its anti-skin aging effect. A gel for local delivery of Codonopsis pilosula water extract was prepared. Firstly，cationic Bletilla striata polysaccharide was prepared by Williamson synthesis method，which was characterized by Fourier transform infrared spectrometer（FTIR），potential，degree of substitution of nitrogen（DS） and other tests. Then，the composite gel matrix（CBSP-CP） was prepared by electrostatic interaction with Carbopol（CP），and its steady-state viscosity，rheological curve and bio-compatibility were evaluated. CBSP-CP gel matrix and three kinds of CP gel matrices were combined with Codonopsis pilosula water extract respectively. [Results] the water extract of codonopsis pilosula can significantly inhibit the transcription activity of AP-1 and has certain anti-skin aging effect on skin. The results of FTIR，potential and DS show that CBSP has been successfully prepared. CBSP-CP composite gel matrix has steady-state viscosity and biocompatibility，and CBSP-CP Codonopsis pilosula aqueous extract gel has better stability in viscosity than three kinds of CP Codonopsis pilosula aqueous extract gels. [Conclusion] This study provides a reference for the development of highly stable gel of traditional Chinese medicine codonopsis pilosula extract，and also provides a way for the application of traditional Chinese medicine codonopsis pilosula extract in anti-skin aging products.