川芎和当归挥发性成分指纹图谱与多成分定量分析及差异比较

Fingerprint and multi-component quantitative analysis and difference comparison of volatile components between Ligusticum Chuanxiong Hort. and Angelica sinensis（Oliv.）Diels

关键词:  川芎  当归  超高效合相色谱  指纹图谱  化学模式识别  含量测定

Key words:  Ligusticum Chuangxiong hort.  Angelica sinensis（Oliv.） Diels  Ultra Performance Convergence Chromatography  fingerprint  chemical pattern recognition  content determination

摘要:

[目的] 建立基于超高效合相色谱（UPC²）的川芎和当归挥发性成分指纹图谱和多成分含量测定方法，比较川芎和当归挥发性成分差异。[方法] 采用超临界流体色谱（SFC），以CO₂为流动相，甲醇为改性剂提取川芎和当归中的挥发性成分；采用Waters Viridis^® BEH色谱柱（3.0×100 mm，130 A，1.7 μm），以CO₂为流动相，0.1%甲酸-甲醇（v/v）为改性剂，建立川芎和当归的指纹图谱和含量测定方法；对两者共有峰进行化学模式识别，鉴别关键差异成分。[结果] 11批川芎中确定18个共有峰，相似度大于0.94；11批当归中确定11个共有峰，相似度大于0.96；通过标准品指认了7个主要共有峰，分别为藁本内酯、洋川芎内酯A、欧当归内酯A、阿魏酸、洋川芎内酯H、阿魏酸松柏酯、洋川芎内酯I；7个化学成分在川芎中的含量均高于当归，其中关键性差异成分为藁本内酯、洋川芎内酯A和阿魏酸松柏酯。[结论] 建立的川芎和当归UPC²指纹图谱和多成分定量分析方法快速、稳定、可靠、环保，可为完善川芎和当归的质量控制提供参考，当归的挥发性成分含量较川芎低，可能是其功效差异的物质基础。

Abstract:

[Objective] To establish an Ultra Performance Convergence Chromatography（UPC²）fingerprint and multi-component content determination method for the volatile components of Ligusticum chuanxiong and Angelica sinensis，and to compare the differences in volatile components between the two herbs. [Methods] Supercritical fluid chromatography（SFC）was used to extract the volatile components from L. chuanxiong and A. sinensis using CO₂ as the mobile phase and methanol as a modifier. A Waters Viridis^® BEH column（3.0×100 mm，130 Å，1.7 μm）was employed to establish the fingerprint and content determination methods，with CO₂ as the mobile phase and 0.1% formic acid in methanol（v/v）as the modifier. Chemical pattern recognition was conducted on the common peaks of the two herbs to identify the key differential components. [Results] Eighteen common peaks were identified in 11 batches of L. chuanxiong，with a similarity greater than 0.94. Eleven common peaks were identified in 11 batches of A. sinensis，with a similarity greater than 0.96. Seven major common peaks were identified by reference standards， namely ligustilide，senkyunolide A，levistolide A，ferulic acid，senkyunolide H，coniferyl ferulate，and senkyunolide I. The contents of these seven chemical components in L. chuanxiong were higher than those in A. sinensis，among which the key differential components were ligustilide，senkyunolide A，and coniferyl ferulate. [Conclusion] The UPC² fingerprint and multi-component quantitative methods for L. chuanxiong and A. sinensis were demonstrated to be rapid，stable，reliable，and environmentally friendly，which can serve as a reference for improving the quality control standards of these two herbs. The lower content of volatile components in A. sinensis than in L. chuanxiong may be the material basis for their differences in efficacy.