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| Content Analysis of Chrysophanol in Cassia Obtusifolia L.by HPLC |
| Hits 1780 Download times 1449 Received:March 20, 2002 |
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| DOI
10.11656/j.issn.1673-9043.2002.03.38 |
| Key Words
HPLC;chrysophanol;Cassia obtusifolia |
| Author Name | Affiliation | | Qi Aidi | Tianjin University of TCM, Tianjin 300193 | | Yan Xuemei | 天津中医学院第一附属医院 300193 | | Wang Hong | Tianjin University of TCM, Tianjin 300193 |
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| Abstract
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| Objective: To find a quantitative determination method by HPLC for examining the effective content of combining chrysophanol in Cassia obtusifolia L.,which could decrease blood pressure,blood lipid and protect the liver.Methods: SUPELCOSILTMLC18Column (5μm,4.6mm×250mm)was used with flow rate1.0 mL/min,detected wave 254 nm,chromatogratic column temperature 40℃ and mobile phase MeOH-5%AxOH(75:25).The content of chrysophanol was detected. Results: The linear relationship was fine (γ=0.9997) in (0.08～3.20)μg. The recovery rate of free chrysophanol was 97.45%,RSD2.21%;of total chrysophanol was102.14%,RSD1.85%.Conclusion: This method is specific and can be used to detect the content of chrysophanol. |
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