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| The Experimental Study of Isolation and Culturating of Bone Marrow Stromal Cell and Differentiation into Cardiomyocyto-Like Cells Induced by Salviandic Acid B in Vitro |
| Hits 2138 Download times 1942 Received:December 10, 2004 |
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| DOI
10.11656/j.issn.1673-9043.2005.01.04 |
| Key Words
salvianolic acid B;marrow stromal cells;induction;cardiac-like muscle cells |
| Author Name | Affiliation | | FAN Ying-chang | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | HUA Sheng-yu | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | YAO Ya-juan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] To discuss the growth and spread of rat marrow stromal cells (rMSCS) in vitro and the probability of Salvianolic acid B induce rMSCS to cardiac-like muscle cells.[Methods] The rMSCS from adult rats,which attached to the walls in cell culture,were isolated and purified for study.The cardiomyogenic induction was started by adding Salvianolic acid B to the culture medium,and the proliferation and differentiation of them were observed.[Results] The rMSCS grew in colonies,toke the appearance of fibroblasts and differentiated into cardiac-like muscle cells after adding Salvianolic acid B. The rate of differentiation approximated 30% in 4 weeks.[Conclusion] Cardiomyocytes can be generated from rMSCS in vitro,and the marrow stromal cell is one of the best resources of donor cardiomvocvtes. |
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