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| Determination of Trans-nerolidol in Inclusion Complex of Volatile Oil from Lignum Dalbergiae Odoriferae with Hydroxypropyl-β-Cyclodextrin by HPLC Method |
| Hits 1686 Download times 1527 Received:February 10, 2006 |
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| DOI
10.11656/j.issn.1673-9043.2006.02.15 |
| Key Words
hydroxypropyl-β-cyclodextrin inclusion complex;volatile oil;Lignum dalbergiae odoriferae;trans-nerolidol;HPLC method |
| Author Name | Affiliation | | WANG Jing | Tianjin University of TCM, Tianjin 300193, China | | ZHANG Tie-jun | Tianjin University of TCM, Tianjin 300193, China | | LIAO Mao-liang | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish a HPLC method for determination of trans-nerolidol in the Inclusion Complex of the volatile oil from Lignum dalbergiae odoriferae with Hydrox-ypropyl-β-Cyclodextrin. [Methods] The HPLC separation was performed on a Diamonsil C,A column (250×4.6 mm, 5 μ) with(olumn temperature at 30℃,a mobile phase consisted of methanol-water (85:15) with a flow rate of 1.0 mL/min and the UV detector wavelength was 210 nm. [Result] The calibration curve was linear in the concentration range of 0.1×10-5~0.6×10-3 μL (r=0.999 9). The mean recovery rate was 102.62% and the RSD was 1.314% (n=9). [Conclusion] This method was simple, rapid, accurate,reproducible and can be used for the quality control of the Inclusion Complex of the volatile oil from Lignum dalbergiae odoriferae with Hydroxypropyl-β-Cyclodextrin. |
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