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| Effect of BushenTiaochong Containing Serum on Expression of Bcl-2,P53 Protein in Isolated Rat Ovary Granular Cells in Vitro |
| Hits 1722 Download times 1847 Received:September 18, 2006 |
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| DOI
10.11656/j.issn.1673-9043.2007.01.11 |
| Key Words
BuShenTiaoChong;ovary granular cells;Bcl-2 oncogene protein;P53 oncogene protein;serum pharmacology |
| Author Name | Affiliation | | LI Pei-lin | The Second Affiliated Hospital to Tianjin University of TCM, Tianjin 300150, China | | HAN Bing | The Second Affiliated Hospital to Tianjin University of TCM, Tianjin 300150, China | | HOU Li-li | The Second Affiliated Hospital to Tianjin University of TCM, Tianjin 300150, China | | 侯莉莉 | 南京市妇幼保健院 210004 |
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| Abstract
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| [Objection] To study the effect of BuShenTiaoChong (Tonifying kidney and regulating CHONG meridian) prescription on the expression of Bcl-2 and P53 proto-oncogene protein in isolated rat ovary granular cells (GC) in vitro.[Methods] Using a method of medicinecontaining serum the cultured in vitro ovary GC was co-cultured with rat serum containing different concentration of medicine for 24 hours. The expression of Bcl-2 and P53 oncogene protein in rat ovary granular cells was examined by immunohistochemistry.[Results] The serum with medicine could increase the positive expression of Bcl-2 oncogene protein in GC.[Conclusion] As the intra-ovarian local regulators Oncogene has involved in the process of apoptosis of GC. The BushenTiaochong containing serum may inhibit apoptosis of GC through promoting the protein expression of anti-oncogene in ovary granular cells. |
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