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| Determination of furocoumarin in the fruit of changium smyrnioides by anti-HPLC |
| Hits 1516 Download times 1815 Received:August 22, 2006 |
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| DOI
10.11656/j.issn.1673-9043.2007.01.14 |
| Key Words
fruit of Changium smyrnioides;furocoumarine;HPLC |
| Author Name | Affiliation | | ZHANG Ping | Nanjing University of TCM, Nanjing 210046, China | | CHEN Jian-wei | Nanjing University of TCM, Nanjing 210046, China | | LI Xiang | Nanjing University of TCM, Nanjing 210046, China |
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| Abstract
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| [Objection] To establish a determining method by HPLC for the main components of fruit of Changium smyrnioides.[Methods] The content was determined using Lichrospher5-C18(250 mm×4.6 mm)column with methanol-water (70:30) as mobile phaseh the column temperature was 30℃h the wave length of detection was 312 nm and the flow rate was 1.0 mL/min.[Results] The liner range of furocoumarine was 0.16～2.56 μg, r=0.999 9. The recovery rate was 99.265 7% and RSD 1.719 9%.[Conclusions] The method is easy and credible with a good linear range. It can be used to determine the content of furocoumarine from the fruit of Changium smyrnioides. |
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