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| Determination of levofloxacin in healthy human plasma by high performance liquid chromatography (HPLC) method |
| Hits 1638 Download times 2114 Received:January 16, 2007 |
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| DOI
10.11656/j.issn.1673-9043.2007.02.11 |
| Key Words
HPLC;Levofloxacin;drug concentration in plasma |
| Author Name | Affiliation | | TIAN Jun-sheng | The TCM Research Centre of Tianjin University of TCM, Tianjin 300193, China | | YANG Jia-feng | The TCM Research Centre of Tianjin University of TCM, Tianjin 300193, China | | WANG Yue-fei | The TCM Research Centre of Tianjin University of TCM, Tianjin 300193, China | | 潘桂湘 | The TCM Research Centre of Tianjin University of TCM, Tianjin 300193, China | | 高秀梅 | The TCM Research Centre of Tianjin University of TCM, Tianjin 300193, China | | 张恒昆 | The TCM Research Centre of Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish a HPLC method for the determ ination of Levofloxacin in human plasma and to determine the concentration of Levofloxacin in hum an plasma after oral taking a single dose 200 mg of Levofloxacin tablet in 20 healthy volunteers.[Methods] Plasma sample was added with internal standard Tinidazole and extracted with chloroform.After taking outthe layerofthe chloroform it was extracted with 0.01 mol/L hydrochloric acid repeatedly and the acid solution wasinjected into the H PLC instrum entfordeterm ination.[Results] There was no internal substance in plasma,which interfered with the determination of the samples.The linear range of medicine-containing blood was between 0.025～6.0 mg/L (r=0.999 5),the lowest quantified limit was 0.025 mg/L.The average recovery of extract was more than 93.2%.All the RSD of precision of intra or inter-batch was less than 6.4% and the accuracy was between 89.7%～103.1%.[Conclusions] The method has a good specificity,can be manipulated conveniently.The obtained result is correct with high sensitivity.So it is a suitable method for the determination of Levofloxacin. |
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