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| Effect of dachengqi granula serum on intestinal intraepithelial lymphocytes proliferation of mice |
| Hits 1334 Download times 1565 Received:April 02, 2007 |
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| DOI
10.11656/j.issn.1673-9043.2007.03.14 |
| Key Words
intestinal intraepithelial lymphocytes;cell proliferation;intracellular Ca2+,Dachengqi granula;medicine serum;archen and 3-methylchrysazin |
| Author Name | Affiliation | | ZHANG Yu-ping | Tianjin University of TCM, Tianjin 300193, China | | FANG Bu-wu | 天津医科大学 300070 | | WU Xian-zhong | 天津市中西医结合急腹症研究所 300100 |
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| Abstract
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| [Objective] To explore proliferation effect and mechanism of Dachengqi granula serum on intestinal intraepithelial lymphocytes (IELs).[Methods] To carry out abstraction,assessment,cultivation IELs of Balb/c mice.Compare to archen and 3-methylchrysazin,to prepare different density of Dachengqi granula serumthat include tosingle and multiple intragastric administration.To observe the effect on proliferation and[Ca2+]I of IELs.[Results] The CD3 masccline cell of IELs was (82.76±2.61),the CD8 masccline cell of IELs was (72.48±3.57),the CD4 masccline cell of IELs was (9.91±2.52).The serum of single and multiple intragastric administration may obviously stimulate the proliferation of IELs.The serum of single intragastric administration was concerned with density,adopting blood time and effect time ofmedicine serum.The equalitymolarityliquid of 3-methylchrysazin had a better effect power.The normal serum of rats,medical serum and PHA may increase IELs[Ca2+]i.[Conclusions] The serum of Dachengqi granula may obviously stimulate IELs proliferation and protect bowels immunologic barrier and one ofeffect mechanismwas the increasion of IELs[Ca2+]i. |
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