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| Effects of Ligustrazine and ferulaic acid on leukocyte-cardiac microvascular endothelial cell adhesion |
| Hits 1656 Download times 1723 Received:February 04, 2007 |
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| DOI
10.11656/j.issn.1673-9043.2007.03.15 |
| Key Words
ligustrazine;ferulic acid;endothelial cell;adhesion;adhesion molecule |
| Author Name | Affiliation | | WANG Jing | Tianjin University of TCM, Tianjin 300193, China | | ZHANG Mi-xia | Tianjin University of TCM, Tianjin 300193, China | | ZHUANG Peng-wei | Tianjin University of TCM, Tianjin 300193, China | | 冯英 | Tianjin University of TCM, Tianjin 300193, China | | 张艳军 | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To study the effects of Ligustrazine and ferulic acid on leukocyte-cardiac microvascular endothelial cell adhesion.[Methods] Human cardiac microvascular endothelial cell (HCMEC) was cultured and hypoxia-reoxgenation model of endothelial cells were performd.The effect of Ligustrazine and ferulic acid on adhesion of leukocyte to activated cardiac microvascular endothelial cells was evaluated by counting.The effects of Ligustrazine and ferulic acid on E-selectin,P-selectin and ICAM-1 mRNA mRNA expression were determined by reverse transcription polymerase chain reaction.[Results] The adhesion rate of leucocyte to HCMECs was increased after hypoxia 2 h-reoxgenation 1 h.When endothelial cells were treated with Ligustrazine or ferulic acid ahead,the adhesion rate was decreased.The expression of P-selectin and ICAM-1 mRNAwere up regulated after hypoxia 2 h-reoxgenation 0.5 h.The expression of P-selectin and ICAM-1 mRNA were up regulated after hypoxia 2 h-reoxgenation 4 h.When endothelial cells were treated with ferulic acid (0.05 mmol/L,0.1 mmol/L) ahead,the expression of E-selectin,P-selectin and ICAM-1 mRNA were down regulated after hypoxia 2 h-reoxgenation 0.5 h.Ferulic acid 0.1 mmol/L can decrease the expression of P-selectin mRNA after hypoxia 2 h-reoxgenation 4 h.While ferulic acid 0.05 mmol/L can decrease the expression of E-selectin mRNA after hypoxia 2 h-reoxgenation 4 h.When endothelial cells were treated with Ligustrazine (0.0625mmol/L) ahead,the expression of E-selectin and P-selectin mRNA were down regulated after hypoxia 2 h-reoxgenation 0.5 h and the expression of E-selectin and ICAM-1 mRNA were down regulated after hypoxia 2 h-reoxgenation 4 h.[Conclusions] Ligustrazine and ferulic acid can inhibit the expression of E-selectin,P-selectin and ICAM-1 mRNA mRNA and leukocyte-endothelial cell adhesion.This maycontfibute toits protective effect against ischemia-reperfusion injury. |
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