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Study of fingerprint of rhizoma corydalis by HPLC |
Hits 1736 Download times 1726 Received:February 12, 2007 |
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DOI
10.11656/j.issn.1673-9043.2007.03.17 |
Key Words
Rhizoma Corydalis;HPLC-fingerprint;Tetrahydropalmatine |
Author Name | Affiliation | DOU Zhi-ying | TianJin University of Traditional Chinese Medicine, Tianjin 300193, China | SUN Wei | TianJin University of Traditional Chinese Medicine, Tianjin 300193, China | ZHANG Min | TianJin University of Traditional Chinese Medicine, Tianjin 300193, China | 田丽莉 | TianJin University of Traditional Chinese Medicine, Tianjin 300193, China |
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Abstract
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[Objective] To set up a method for HPLC-fingerprint of Rhizoma Corydalis in Shanxi.The conclusion makes reference to standard of quality control of Rhizoma Corydalis.[Methods] The methods were performed by RP-HPLC.Kromasil ODS-C18 Column was used at 30℃,acetonitrile-acetate buffer solution (pH 6.0) as mobile phase,gradient eluting,flow rate was 1.0 mL/min,detection wavelength was set at 280 nm and eluting period were 75 min.[Results] HPLC-fingerprint of Rhizoma Corydalis was established,which contained 17 common peaks.[Conclusions] The established fingerprint can be used for the quality control and species identifying of Rhizoma Corydalis. |
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