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| Determination of monoamine neurotransmitter and its metabolites by high performance liquid chromatography and electrochemical detection |
| Hits 1572 Download times 2097 Received:May 02, 2007 |
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| DOI
10.11656/j.issn.1673-9043.2007.04.09 |
| Key Words
HPLC-ECD;Monoamine Neurotransmitter;Metabolites |
| Author Name | Affiliation | | YANG Jia-feng | Key Laboratory of Pharmacology of TCM Formulae, Ministry of Education Research Center of TCM, Tianjin University of TCM, Tianjin 300193, China | | LI Juan | Key Laboratory of Pharmacology of TCM Formulae, Ministry of Education Research Center of TCM, Tianjin University of TCM, Tianjin 300193, China | | YANG Hong | Key Laboratory of Pharmacology of TCM Formulae, Ministry of Education Research Center of TCM, Tianjin University of TCM, Tianjin 300193, China | | 潘桂湘 | Key Laboratory of Pharmacology of TCM Formulae, Ministry of Education Research Center of TCM, Tianjin University of TCM, Tianjin 300193, China | | 高秀梅 | Key Laboratory of Pharmacology of TCM Formulae, Ministry of Education Research Center of TCM, Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] A simple, rapid and sensitive HPLC-ECD method was established to detect the monoamine neurotransmitter and its metabolites. [Methods] The LC was performed on a Waters @ ODS C18 column (3.9×500 mm, 5μm). The mobile phase, consisted of Methanol-Acetonitrile-Water phase (containing B 8 1.25 g, KH2PO4 6.8 g, EDTA 18 mg in 500 mLsolution, pH=3.3)=19:3:78. The mobile phase was at a flow-rate of 0.8 mL/min. The electrochemical detector was equipped with a glass carbon working electrode and a Ag/AgCl reference electrode. The working volt was 0.7 V. The column temperature was kept at 35℃. [Results] Their retention time were 3.6 min for NE, 4.5 min for 5-HIAA, 5.2 min for HVA, 6.1 min for DA and 11.9 min for 5-HT respectively. These five components were all found tobe linear over a concentration range of 20～1 000 μg/L. The lowlimit of quantitation was 4～8 μg/L. [Conclusion] The HPLC-ECD method was rapid, sensitive and specific for quantitative deter-mination ofmonoamine neurotransmitter and its metabolites. |
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