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| In vivo pharmaco kinetics study of total curcumine in rats |
| Hits 1607 Download times 1888 Received:June 20, 2008 |
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| DOI
10.11656/j.issn.1673-9043.2008.04.13 |
| Key Words
total curcumine;chromatography;liquid-liquid extraction |
| Author Name | Affiliation | | ZHU Qing-hua | Tianjin University of TCM, Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin 300193, China | | LIU Cai-xia | Tianjin University of TCM, Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin 300193, China | | CHEN Jing | Tianjin University of TCM, Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin 300193, China | | 朱晓薇 | Tianjin University of TCM, Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin 300193, China | | 国大亮 | Tianjin University of TCM, Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin 300193, China |
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| Abstract
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| [Objective] To carry out a in vivo pharmacokinetics study on total curcumine and establish a HPLC determination method of blood curcumine.[Methods] The plasma of total curcumine was prepared and the content of total curcumine determined.Using Hypersil C18 column (250 mm×4.6 mm,5 um),the mobile phase was a mixture of acetonitrile-acetic acid (46:54) and the wavelength was 420 nm.[Results] The line range of total curcumine was 2~300 ug/L,the lowest limit of quantity was 2 ug/L and RSD was between 5.08% and 7.93%. [Conclusion] The method is simple,accurate and can be used to control the quality of total curcumine and in pre-clinic pharmacokinetics study. |
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