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| Determination of paeoniflorin in Naoxueshuan pill (Yihao) by HPLC method |
| Hits 1816 Download times 1816 Received:December 20, 2008 |
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| DOI
10.11656/j.issn.1673-9043.2009.02.12 |
| Key Words
Naoxueshuan Pill(Yihao);Paeoniflorin;HPLC;Content determination |
| Author Name | Affiliation | | ZHANG Li-ling | The Shitian Medicine Limited-liability company in Tianjin, Tianjin 300382, China | | GUO Wei | 天津市中医药大学第一附属医院 300193 |
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| Abstract
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| [Objective] To establish a HPLC method to determine the content of Paeoniflorin in Naox-ueshuan Pill (Yihao).[Methods] The chromatographic column was Inertex C18 (5μm,250 mm×4.6 mm).The mobile phase was acetonitrile-water (15:85).The flow rate was 1.0 mL/min and the detection wavelength was 230 nm.[Results] Linearity range of Paeoniflorin was 0.608~3.04μg (r=0.999 9). The average recovery was 102.7%,and RSD was 2.0%.[Conclusion] This method was accurate,reliable and reproducible.It can be used for assaying Paeoniflorin in Naoxueshuan Pill (Yihao). |
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