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| Determination of Chrysophanol in Pedo-digestion & fever- bringing-down Syrup by HPLC |
| Hits 1749 Download times 1669 Received:November 20, 2008 |
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| DOI
10.11656/j.issn.1673-9043.2009.02.13 |
| Key Words
HPLC;Digestion&bringing down a fever syrup;Chrysophanol determination |
| Author Name | Affiliation | | WANG Shu-bei | Department of Pharmacy, GeneraL HospitaL of Tianjin Medical University, Tianjin 300052, China | | YUAN Heng-jie | Department of Pharmacy, GeneraL HospitaL of Tianjin Medical University, Tianjin 300052, China | | QI Ai-di | 天津中医药大学 300193 |
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| Abstract
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| [Objective] To establish HPLC method for determination of chrysophanol in pedo-digestion &fever-bringing-down syrup.[Method] The determination was performed on C18 column,the mobile phase consisted of methanol-water-phosphoric acid-sodimn pentanesulfonic acid (80:20:0.1:0.1 g) with flow rate at 1.0 mL/min and detection wavelength at 254 nm under room tempterature.[Results] Chrysophanol had good linear relation in the range of 5～25 mg/L (r=0.999 9),the average recovery was 100.13% (RSD=1.1%).[Conclusion] The method can be used to control the quality of digestion& bringing down a fever syrup. |
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