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| Preparation of psoralenoside and isopsoralenoside by preparative high performance liquid chromatography from psoralen |
| Hits 1883 Download times 1989 Received:June 11, 2009 |
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| DOI
10.11656/j.issn.1673-9043.2009.03.15 |
| Key Words
psoralenoside;isopsoralenoside;preparative HPLC |
| Author Name | Affiliation | | GUO Jin-ming | Tianjin University of TCM, Tianjin 300193, China | | LIU Ya-nan | Tianjin University of TCM, Tianjin 300193, China | | WANG Yue-fei | 天津中医药大学中医药研究院, 现代中药发现与制剂技术教育部工程研究中心 300193 | | QI Ai-di | 天津中医药大学中医药研究院, 现代中药发现与制剂技术教育部工程研究中心 300193 | | 韩立峰 | 天津中医药大学中医药研究院, 现代中药发现与制剂技术教育部工程研究中心 300193 |
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| Abstract
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| [Objective] To establish a method for isolation and purification of psoralenoside and isopsoralenoside by preparative HPLC.[Methods] After being extracted with ethyl acetate, the seeds of Psoralea corylifolia was extracted by methanol. Then the methanol extracts were evaporated to dryness under reduced pressure. The extract was resuspended by water and separated via AB-8 type macroporous resin column chromatography, then eluted by distilled water. Fraction collected was concentrated to a certain volume. The solution was isolated and purified by preparative HPLC. The method was performed on a Hanbon preparative column C18 （20 mm×250 mm,5 μm）using the elution of acetonitrile and water containing 0.05% formic acid （15:85）at room temperature. The flow rate was 8.0 mL/min and the wavelength of measurement was 246 nm. [Resutls] Two compounds were isolated from the seeds of Psoralea corylifolia. [Conclution] The purity of the product quantitated by normality was over 96%. The developed method is simple and rapid, which could be used for preparation of psoralenoside and isopsoralenoside. |
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