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| Experimental study on differentiation of bone marrow mesenchymal stem cells into myocardium-like cells induced by conditioned medium |
| Hits 2051 Download times 1723 Received:August 20, 2009 |
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| DOI
10.11656/j.issn.1673-9043.2009.04.10 |
| Key Words
Conditioned medium;XinFuKang;MSCs;Immunocytochemistry;Real time fluorescent quantified RT-PCR |
| Author Name | Affiliation | | XU Xiu-mei | Tianjin Yongjiu Hospital, Tianjin 300451, china | | JIANG Xi-juan | 天津中医药大学 300193 | | ZHOU Bing | Tianjin Yongjiu Hospital, Tianjin 300451, china | | 郭茂娟 | 天津中医药大学 300193 | | 马志刚 | Tianjin Yongjiu Hospital, Tianjin 300451, china | | 范英昌 | 天津中医药大学 300193 |
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| Abstract
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| [Objective] To observe the effect of conditioned medium in inducing the bone marrow mesenchymal stem cells (MSCs) differentiation into myocardium-like cells.[Methods] MSCs were cultured,purified and identified.They were induced by pure conditioned medium (TJ),conditioned medium 1 (TJ-ZY) and conditioned medium 2 (TJ-XFK) respectively.After that they were cultured for four weeks and evaluated the expression of cardiac troponin T by immunocytochemistry.The expression of the early gene of cardiac muscle NKX2.5,GATA-4,Desmin and a-actin mRNA was evaluated by real time fluorescent quantify RT-PCR.[Results] After inducing,the pure conditioned medium group doesn't express cTnT,but the Xinfukang group does.All groups expressed the four early gene of cardiac muscle,but the expression of the genes in the Xinfukang intervention groups was much more than that in the pure conditioned medium group.[Conclusion] Microenvironment plays an important role in the process of MSCs induced into myocardium-like cells,the Xinfukang can improve the differentiation and specialization of MSCs into myocardium-like cells. |
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