|
| Study on the HPLC/DAD/ELSD fingerprint in intermediates of Danqi Capsule for Hemiplegia |
| Hits 2481 Download times 1944 Received:June 26, 2009 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2009.04.12 |
| Key Words
Hemiplegia Danqi Capsule;Fingerprint;HPLC/DAD/ELSD;Chinese medicine |
| Author Name | Affiliation | | MA Zhi-ping | Tianjin University of TCM, Tianjin 300193, China | | CHEN Tao | 天津中医药大学第一附属医院 300193 | | LI Jin | 天津中医药大学第一附属医院 300193 | | 孟萌 | Tianjin University of TCM, Tianjin 300193, China | | 贾薇 | Tianjin University of TCM, Tianjin 300193, China | | 贾美娜 | Tianjin University of TCM, Tianjin 300193, China |
|
| Abstract
|
| [Objective] To establish the HPLC/DAD/ELSD fingerprint of Danqi Capsule for hemiplegia for the full scientific control of Danqi capsule hemiplegia and to provide a new method for the quality control.[Methods] The technique in series of HPLC/DAD/ELSD was applied.Waters Bridge,RP18 (4.6 mm×250 mm,5.0μm) were the column.The mobile phase:A:10%acetonitrile-aceticacid (500:2.2,v/v),B:10%acetonitrile-acetic acid (500:2.2,v/v).The flow rate was 1 mL/min,Wavelength detection at 254 nm,Drift tube temperature was 50℃,Heater level was 68%,Carrier gas pressure was 35PSI,Add value to 60.[Results] Based on the method established in the Experiment UV absorption and UV-free ingredients in Hemiparalysis Dan Capsules were well-separated,At the same time a sample was determined by two different components of the fingerprint,The similarity between different batches was able to meet the requirements.[Conclusion] This method can be used for hemiplegia Danqi Capsule intermediates in two different components of the fingerprint measured at the same time. |
|
|
|
|
|
