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| Determination of total saponins in anti-inflammatory and antasthmatic extractum |
| Hits 2424 Download times 1935 Received:May 31, 2009 |
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| DOI
10.11656/j.issn.1673-9043.2009.04.13 |
| Key Words
anti-inflammatory and antasthmatic extractum;total saponins;quality control |
| Author Name | Affiliation | | ZHAO Qi-duo | Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica, Tianjin University of TCM, Tianjin 300193, China | | WANG Ying | Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica, Tianjin University of TCM, Tianjin 300193, China | | SHU Le-xin | Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica, Tianjin University of TCM, Tianjin 300193, China | | 宋志国 | 吉林敖东集团大连药业股份有限公司 116043 | | 李金梅 | 吉林敖东集团大连药业股份有限公司 116043 |
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| Abstract
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| [Objective] To establish a method for Determining the total Saponins in anti-inflammatory and antasthmatic extractum.[Methods] Total saponins were extracted with ultrasonic extraction technique in methanol and purified by normal butanol extracting.The method of Vanillin-Sulfuric acid spectrophotometry was used to determine total saponins taken ginsenoside Rb1 as the standard sample. [Results] The detection wavelength was 552nm and the standard curve (C=168.3A+2.846) was linear in the range of 10-160μL r=0.999 4).The average recovery rate was 99.9 1%and RSD was 0.60%. [Conclusion] The method was feasible and accurate for the quality control of the extractum. |
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