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| Effect of PNS on expression of apoptosis-relating mRNA and protein in impaired CNC caused by hypoxia/re-oxygenation |
| Hits 2386 Download times 2365 Received:December 01, 2009 |
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| DOI
10.11656/j.issn.1673-9043.2010.01.09 |
| Key Words
PNS apoptosis;Bax;Bcl-2;caspase-3;mRNA;protein |
| Author Name | Affiliation | | KANG Li-yuan | Tianjin University of Traditional Chinese Medicine, Tianjin300193, China | | ZHOU Zhi-huan | Tianjin University of Traditional Chinese Medicine, Tianjin300193, China | | ZHANG Meng | Tianjin University of Traditional Chinese Medicine, Tianjin300193, China | | 柴丽娟 | Tianjin University of Traditional Chinese Medicine, Tianjin300193, China | | 高秀梅 | Tianjin University of Traditional Chinese Medicine, Tianjin300193, China | | 王怡 | Tianjin University of Traditional Chinese Medicine, Tianjin300193, China | | 郭红 | Tianjin University of Traditional Chinese Medicine, Tianjin300193, China | | 闫晨 | Tianjin University of Traditional Chinese Medicine, Tianjin300193, China |
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| Abstract
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| [Objective] In order to research the protective effect of PNS against apoptosis, we observed the effects of PNS on the mRNA expression of Bcl-2, Bax, caspase-3 and caspase-3 activity in impaired CNC model caused by hypoxia/re-oxygenation.[Methods] We cultured cortical neuronal cell (CNC) of rat in vitro and established an impaired cell model by hypoxia/re-oxygenation (H/R). We treated the impaired cells with three dosages, 50 mg/L, 10 mg/L, 2 mg/L of PNS, and studied the mRNA expression of Bcl-2, Bax, caspase-3 by the method of real time RT-PCR. We studied the protein activity of caspase-3 by the method of Lowrry.[Results] 50 mg/L, 10 mg/L of PNS decreased the expression of Bax significantly compared with H/R group. 50 mg/L of PNS increased the expression of Bcl-2 significantly compared with H/R group. H/R group had a tendency of up-regulating and PNS group had a tendency of down-regulating the expression of caspase-3. These three dosages of PNS inhibited the protein activity of caspase-3 with a tendency of dose -dependent manner.[Conclusion] The possible protective mechanism of PNS against apoptosis may be by decreasing the expression of Bax, increasing the expression of Bcl-2 and inhibiting the protein activity of caspase-3. |
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