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| Study on effect of Salvianolic acid B on apoptosis of myocardial cells after acute myocardial infarction in rats |
| Hits 1924 Download times 1795 Received:December 11, 2009 |
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| DOI
10.11656/j.issn.1673-9043.2010.02.10 |
| Key Words
Salvianolic acid B;apoptosis;LN;PⅢNP;Integrin;FAK |
| Author Name | Affiliation | | MENG Qing-nan | | | LI Guang-bin | | | GENG Xiao-li | | | 郭茂娟 | | | 范英昌 | |
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| Abstract
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| [Objective] To study the effect and mechanism of Salvianolic acid B (SalB) on the apoptosis of myocardial cells after acute myocardial infarction (AMI) in Rats.[Methods] Myocardial infarction was induced by ligating the descending left coronary artery in rats.The rats were divided into sham-operated group,model group and Sal B group.Sal B was given with 120 mg/ (kg·d) by gastric perfusion for 4 weeks after infarction in Sal B group.The animals in each group were killed after 1 week,2 weeks and 4 weeks of operation respectively.Radioimmunoassay was used to measure the content of laminin (LN) and ProcollagenⅢN-terminal Peptide (PⅢNP) in serum of rats;Masson staining was used to determine collagen volume fraction (CVF) in non-infarcted regions;Immunohistochemical method was applied to examine the expression of integrin and focal adhesion kinase (FAK) in myocardial tissues;TUNEL was used to detect the changes of apoptosis of myocardial cells.[Results] Compared with sham-operated group,content of LN and PⅢNP in serum increased (P<0.01) 1 week,2 weeks and 4 weeks after operation in model group,CVF increased,expression of integrin and FAK decreased,myocardial apoptosis index increased (P<0.05) ;compared with model group,content of LN and PⅢNP in serum was reduced (P<0.01) 1 week,2 weeks and 4 weeks after operation in Sal B group,CVF was reduced,expression of integrin and FAK was increased,apoptosis of myocardial cells index was reduced (P<0.05).[Conclusion] Sal B can reduce the apoptosis of myocardial cells after AMI in rats,and its mechanism may be related to reducing the content of LN and PⅢNP in serum,reducing changes of collagen and extracellular matrix in myocardial tissue,and activating integrin and FAK signaling pathway. |
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