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| Effects of ginkgolide B on serum CRP and aortic VCAM-1 mRNA in rabbit with atherosclerosis |
| Hits 1851 Download times 1729 Received:January 08, 2010 |
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| DOI
10.11656/j.issn.1673-9043.2010.02.11 |
| Key Words
Atherosclerosis;Ginkgolide B;CRP;VCAM-1 |
| Author Name | Affiliation | | MA Yi-wen | | | MA Dong-ming | | | LU Bin | | | 姜希娟 | |
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| Abstract
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| [Objective] To explore the effect of ginkgolides B on the content of serum CRP and the expression of aortic VCAM 1-gene in rabbits with atherosclerosis.[Methods] High fat feed was use to induce rabbit model of atherosclerosis.Thirty-five rabbits were divided into control (ordinary),model group,and ginkgolides B group,the last two groups were given high fat feed for eight weeks.After that the rabbits in ginkgolides B group were fed with EGB,5 mg/ (kg·d).They were continued to feed for another eight weeks.Blood sample was extracted from ear margin vein before death.Enzyme-linked immunosorbent assay (ELISA) was used to detect the rabbit serum CRP levels.After death,a sample of aortic artery was taken to detect VACM-1 gene expression by PCR method.[Results] The CRP levels of model group was obviously higher than that of control group;serum CRP content of ginkgolides B group was lower than that of model group.There was a significant difference between both groups (P<0.01).VCAM-1 gene expression in model group was higher than control,ginkgolides B group was lower than model group,obviously difference was found between two groups (P<0.01).[Conclusion] Ginkgolides B can reduce serum CRP levels in rabbits with atherosclerosis,inhibit the aortic VCAM-1 gene expression effectively,thus Preventing the development of arteriosclerosis. |
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