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Screening of mother medium and the optimal media composition for the liquid fermentation of fomitopsis pinicola |
Hits 1713 Download times 1768 Received:November 13, 2009 |
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DOI
10.11656/j.issn.1673-9043.2010.02.15 |
Key Words
Fomitopsis pinicola;polysaccharide;mother medium;liquid fermentation;orthogonal test |
Author Name | Affiliation | Xu Tian-tian | | Ma Lin | |
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Abstract
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[Objective] To select the mother medium composition and the optimal media composition for the liquid fermentation of Fomitopsis pinicola.[Methods] The same amount of Fomitopsis pinicola was cultured to compare their growth rate and aging degree by four different solid media.Taking the mycelial biomass and production of polysaccharide as the detecting indices,the optimum submerged fermentation parameters for Phellinus igniarius were studied by single factor test and orthogonal test.[Results] Fomitopsis pinicola growing on chemical media grew quickly,so the chemical media was suitable to be used as expanding species for reproduction.The aging degree of hypha growing on chemical media was most slowly,so bran media was suitable to be used as conservation.The optimum formula for liquid fermentation (g/100mL) were:rise powder 5,yeast 1,KH2POSUB>3 0.1,MgSOSUB>4 0.15,VB1 0.040.[Conclusion] Our study has selected the mother medium composition and the optimal media composition for the liquid fermentation of Fomitopsis pinicola and provided the scientific basis for the further research of Fomitopsis pinicola. |
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