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| Determination of jasminoidin in ZhiHuang Eye Drops by HPLC method |
| Hits 1374 Download times 1772 Received:June 11, 2010 |
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| DOI
10.11656/j.issn.1673-9043.2010.03.13 |
| Key Words
Eye drops;Jasminoidin;HPLC;Content determination |
| Author Name | Affiliation | | GUO Wei | The First Hospital Affiliated to Tianjin University of TCM, Tianjin 300193, China | | ZHAO Hui-ping | The First Hospital Affiliated to Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish a HPLC method for determination the content of jasminoidin in ZhiHuang Eye Drops.[Methods] The chromatographic column was Inertex C18 (4.6 mm×250 mm,5μm).The mobile phase was acetonitrile-water (13:87).The flow rate was 1.0 mL/min and the detection wavelength was 238 nm.[Results] Linearity range of jasminoidin was 0.688-3.44μg (r=0.9999).The average recovery was 99.1%,RSD was 1.1% (n=6).[Conclusion] The method is simple,accurate and reproducible.It can be used for assaying jasminoidin in ZhiHuang Eye Drops. |
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