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| Optimization of HPLC conditions for Chlorogenic Acid in Shurening mixture |
| Hits 1702 Download times 1686 Received:October 20, 2010 |
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| DOI
10.11656/j.issn.1673-9043.2011.01.18 |
| Key Words
HPLC；Shurening mixture;Lonicera japonica Thunb;Chlorogenic Acid |
| Author Name | Affiliation | | HU Xiaoqin | Tianjin University of TCM, Tianjin 300193, China
| | LI Jin | The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China | | CHEN Tao | The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China | | 孟萌 | The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To optimize the chromatographic conditions for determining Chlorogenic Acid in Shurening (Summer Heat Ning) mixture.[Methods] Xterra188 Waters RP C18 (250mm×4.6mm,5μm) column was used;Detecter was waters2998;Work station:Waters Empower;The motile phase:acetonitrile-0.4% Phosphoric acid (10∶90), The flow rate was 1 mL.per min,and the detection wavelength was set at 327nm.[Results] This condition could have a good separation for Chlorogenic Acid.[Conclusion] It was a simple,accurate,fast and inexpensive analysis for the adenosine phosphates. |
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