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| Effect of the monomer of traditional Chinese medicine on the lipid-related genes expression in hepatic cell line of HepG2 |
| Hits 1441 Download times 1840 Received:February 11, 2011 |
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| DOI
10.11656/j.issn.1673-9043.2011.02.11 |
| Key Words
salvianolic acid B;curcumine;nuciferine;gold theragan base;lipid-related genes;RT-PCR |
| Author Name | Affiliation | | WANG Wei | Tianjin University of TCM, Tianjin 300193, China | | WANG Zi-xu | Tianjin University of TCM, Tianjin 300193, China | | TIAN Wei | Tianjin University of TCM, Tianjin 300193, China | | 史红 | 天津中医药大学中医药研究中心 300193 | | 江海涛 | 天津中医药大学第二附属医院 300150 |
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| Abstract
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| [Objective] To study the effect of monomer of traditional Chinese medicine on the lipid-related genes expression (PPARα, HL, ApoB100, ApoAII and ApoCII) on the hepatic cell line of HepG2. [Methods] HepG2 cells in vitro were chosen as the study object. They were divided into 5 groups:salvianolic acid B group, curcumine group, nuciferine group, gold theragan base group and normal group. The abundance of PPARα, HL, ApoB100, ApoAⅡ and ApoCⅡ mRNA in hepatic cell line of HepG2 were measured by RT- PCR with β-actin as the internal standard. [Results] Salvianolic acid B, curcumine and nuciferine could enhance the expression of PPARα mRNA;salvianolic acid B could enhance the expression of the HL mRNA;salvianolic acid B, curcumine, nuciferine and gold theragan base could enhance the expression of the ApoB100 mRNA;curcumine gold theragan could enhance the expression of the ApoAII mRNA;salvianolic acid B and gold theragan base could enhance the expression of the ApoCII mRNA. [Conclusion] Shugandaozhuo medicines have effects on fatty liver by increasing the expression of lipid-related genes. |
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