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| Simultaneous determination of luteolin-7-O-glu-coside and chlorogenic acid in Flos Lonicerae Japonicae by HPLC |
| Hits 1972 Download times 2189 Received:February 11, 2011 |
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| DOI
10.11656/j.issn.1673-9043.2011.02.14 |
| Key Words
HPLC;Flos Lonicerae Japonicae;chlorogenic acid;luteolin-7-O-glucoside;quantitative determination |
| Author Name | Affiliation | | ZHANG Yuan-yuan | Tianjin University of TCM, Tianjin 300193, China | | LI Jin | The First Hospital Affiliated to Tianjin University of TCM, Tianjin 300193, China | | CHEN Tao | The First Hospital Affiliated to Tianjin University of TCM, Tianjin 300193, China | | 李凤丽 | The First Hospital Affiliated to Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish a HPLC method for quantitative determination of luteolin-7-O-glu-coside and chlorogenic acid in Flos Lonicerae Japonicae using gradient elution method. [Methods] Determining column:Xterra@ RP 185;Detector:Waters 2 998 diode array detector (DAD);Workstation:Waters Empower;The mobile phase:0.5% acetic acid-acetonitrile;Linear gradient elution:0~15min (90:10-80:20),15~30 min (80:20-75:25), 30~40 min (75:25-65:35), 40~45 min (65:35-90:10);The flow rate was 1 mL/min, and the detection wavelength was set at 350 nm. [Results] In this chromatographic conditions, the two components were completely separated. [Conclusion] The method is simple, accurate, fast and easy, which can detect two components of Flos Lonicerae Japonicae simultaneously. |
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