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| Determination of the concentration of aristolochic acid A in cell medium with HPLC-UV |
| Hits 1649 Download times 2255 Received:November 15, 2010 |
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| DOI
10.11656/j.issn.1673-9043.2011.02.15 |
| Key Words
HPLC-UV method;aristolochic acid A;stability |
| Author Name | Affiliation | | WANG Li | Tianjin University of TCM, Tianjin 300193, China | | ZHOU Hui-fang | Tianjin University of TCM, Tianjin 300193, China | | ZHANG Yan-jun | Tianjin University of TCM, Tianjin 300193, China | | 边育红 | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish the HPLC-UV method for determination of the concentration of aristolochic acid A. [Methods] Samples was precipitated by methanol and centrifugated for 5 min (10 000 r/min). Using HPLC-UV method the samples was determined, and the chromatographic system was consisted of Waters C 18 column (150 nm×4.6 nm,5 μm),with 3% acetic acid water solution-methanol (30:70) at the flow rate of 1.0 mL/min. The detection wavelength was 260 nm and column temperature was 30℃. [Results] The aristolochic acid A showed a good linear relationship at the range of 0.5~50 mg/L (r=0.999 8). The intra-run and inter-run validation were 1.92%, 0.96%. The recovery rate were 94.01%~104.34%. [Conclusion] The samples are stabile in 3 days. The method is high specific and sensitive, simple and reliable, so it can be used to study the concentration of aristolochic acid A in culture medium. |
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